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Model based fed batch cultivation and elicitation for the overproduction of ajmalicine from hairy roots of Catharanthus roseus

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Title Model based fed batch cultivation and elicitation for the overproduction of ajmalicine from hairy roots of Catharanthus roseus
 
Creator Thakore, Dhara
Srivastava, Ashok K.
Sinha, Alok Krishna
 
Subject Ajmalicine
Modelling
Fed batch culture
Kinetic parameters
Elicitation
Plant cell culture
 
Description Accepted date: 3 February 2015
Batch cultivation of Catharanthus roseus hairy roots was attempted in a statistically optimized nutrient
medium. This resulted in a biomass growth of 5.12 ± 0.46 g/l and ajmalicine content of 24.9 ± 1.2 mg/l
on day 30 beyond which the product concentration declined. Using the observed batch kinetics data a
mathematical model was developed which could describe the experimental observations to the extent
of 99% accuracy as demonstrated by the statistical validity test. The model was thereafter extrapolated
to fed-batch cultivation to identify suitable nutrient feeding strategies (by off-line simulations) for the
overproduction of ajmalicine. Eventually the experimental implementation of three such feeding strategies and comparison with the model predictions further reinforced the efficacy of the mathematical
model under dynamic fed batch conditions. A 60% increase in ajmalicine overall productivity and a 2.5
fold increase in volumetric yield was obtained by the “increasing feed rate strategy” as opposed to batch
cultivation. To further improve the alkaloid accumulation a combination of statistically optimized mixture of elicitor(s) (jasmonic acid, methyl jasmonate and KCl) was added after the termination of model
based increasing fed batch cultivation which resulted in a significantly high ajmalicine concentration
of 123.2 ± 8.63 mg/l (a 4 fold increase compared to batch). This is the first report of such a strategy for
overproduction of ajmalicine in rather complex hairy root cultivation.
 
Date 2016-01-25T08:58:37Z
2016-01-25T08:58:37Z
2015
 
Type Article
 
Identifier Biochemical Engineering J., 97: 73-80
1369-703X
http://172.16.0.77:8080/jspui/handle/123456789/586
http://www.sciencedirect.com/science/article/pii/S1369703X1500039X
10.1016/j.bej.2015.02.005
 
Language en_US
 
Publisher Elsevier B.V.