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Magnaporthe oryzae aminosugar metabolism is essential for successful host colonization

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Title Magnaporthe oryzae aminosugar metabolism is essential for successful host colonization
 
Creator Kumar, Anil
Ghosh, Sumit
Bhatt, Dharmendra Nath
Narula, Alka
Datta, Asis
 
Subject Magnaporthe oryzae
 
Description Accepted date: 3 JAN 2016
Pathogens encounter and metabolize a range of host-derived metabolites while proliferating inside the host. Our understanding of these metabolites and their metabolic processes has remained largely incomplete. We investigated the role of the Magnaporthe oryzae N-acetylglucosamine (GlcNAc) catabolic pathway during rice infection. The catabolic pathway is composed of a GlcNAc transporter (MoNgt1), hexokinase(s), a GlcNAc-6-phosphate deacetylase (MoDac) and a GlcN-6-phosphate deaminase (MoDeam). A detailed characterization of the Δmongt1, Δmodac and Δmodeam null mutants revealed that a defect in GlcNAc catabolism impairs the pathogenicity of M. oryzae. These mutants showed severely reduced virulence in susceptible rice cultivar due to their inability to neutralize host-derived reactive oxygen species and their failure to develop invasive hyphal growth within the host tissue. Interestingly, during oxidative stress, M. oryzae proliferated efficiently in GlcNAc-containing media compared to other sugars, and the expression of fungal antioxidant genes was up-regulated following GlcNAc treatment. However, GlcNAc inhibited the growth of the Δmodac and Δmodeam mutants, and this growth inhibition was enhanced during oxidative stress. These results suggest that GlcNAc helps fungus to overcome oxidative stress inside its host, perhaps by activating an anti-oxidant defence. In the absence of a functional catabolic pathway, GlcNAc becomes toxic to the cells.
 
Date 2016-01-28T09:35:02Z
2016-01-28T09:35:02Z
2016
 
Type Article
 
Identifier Environ. Microbiol., 18(3): 1063-1077
1462-2920
http://172.16.0.77:8080/jspui/handle/123456789/603
http://onlinelibrary.wiley.com/wol1/doi/10.1111/1462-2920.13215/abstract
10.1111/1462-2920.13215
 
Language en_US
 
Publisher John Wiley & Sons