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Improving nutritional quality and fungal tolerance in soya bean and grass pea by expressing an oxalate decarboxylase

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Title Improving nutritional quality and fungal tolerance in soya bean and grass pea by expressing an oxalate decarboxylase
 
Creator Kumar, Vinay
Chattopadhyay, Arnab
Ghosh, Sumit
Irfan, Mohammad
Chakraborty, Niranjan
Chakraborty, Subhra
Datta, Asis
 
Subject Glycine max
Lathyrus sativus
oxalic acid
oxalate decarboxylase
Sclerotinia resistance
 
Description Accepted date: 26 October 2015
Soya bean (Glycine max) and grass pea (Lathyrus sativus) seeds are important sources of dietary proteins; however, they also contain antinutritional metabolite oxalic acid (OA). Excess dietary intake of OA leads to nephrolithiasis due to the formation of calcium oxalate crystals in kidneys. Besides, OA is also a known precursor of β-N-oxalyl-L-α,β-diaminopropionic acid (β-ODAP), a neurotoxin found in grass pea. Here, we report the reduction in OA level in soya bean (up to 73%) and grass pea (up to 75%) seeds by constitutive and/or seed-specific expression of an oxalate-degrading enzyme, oxalate decarboxylase (FvOXDC) of Flammulina velutipes. In addition, β-ODAP level of grass pea seeds was also reduced up to 73%. Reduced OA content was interrelated with the associated increase in seeds micronutrients such as calcium, iron and zinc. Moreover, constitutive expression of FvOXDC led to improved tolerance to the fungal pathogen Sclerotinia sclerotiorum that requires OA during host colonization. Importantly, FvOXDC-expressing soya bean and grass pea plants were similar to the wild type with respect to the morphology and photosynthetic rates, and seed protein pool remained unaltered as revealed by the comparative proteomic analysis. Taken together, these results demonstrated improved seed quality and tolerance to the fungal pathogen in two important legume crops, by the expression of an oxalate-degrading enzyme.
This work was financially supported by a research grant (BT/01/
CEIB/12/II/01) from the Department of Biotechnology and core
research grant from the National Institute of Plant Genome
Research. The Central Instrumentation facility at the National
Institute of Plant Genome Research is acknowledged. VK and SG
have received fellowship from the Council of Scientific and
Industrial Research. AC and MI have received fellowship from the
National Institute of Plant Genome Research and the Department
of Biotechnology, respectively.
 
Date 2016-01-28T09:44:20Z
2016-01-28T09:44:20Z
2016
 
Type Article
 
Identifier Plant Biotechnol. Journal, 14(6): 1394-1405
1467-7644
http://172.16.0.77:8080/jspui/handle/123456789/604
http://onlinelibrary.wiley.com/wol1/doi/10.1111/pbi.12503/abstract
10.1111/pbi.12503
 
Language en_US
 
Publisher John Wiley & Sons