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Evolution of disorder in Mediator complex and its functional relevance

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Title Evolution of disorder in Mediator complex and its functional relevance
 
Creator Nagulapalli, Malini
Maji, Sourobh
Dwivedi, Nidhi
Dahiya, Pradeep
Thakur, Jitendra K.
 
Subject Mediator complex
 
Description Accepted date: October 18, 2015
Mediator, an important component of eukaryotic transcriptional machinery, is a huge multisubunit complex. Though the complex is known to be conserved across all the eukaryotic kingdoms, the evolutionary topology of its subunits has never been studied. In this study, we profiled disorder in the Mediator subunits of 146 eukaryotes belonging to three kingdoms viz., metazoans, plants and fungi, and attempted to find correlation between the evolution of Mediator complex and its disorder. Our analysis suggests that disorder in Mediator complex have played a crucial role in the evolutionary diversification of complexity of eukaryotic organisms. Conserved intrinsic disordered regions (IDRs) were identified in only six subunits in the three kingdoms whereas unique patterns of IDRs were identified in other Mediator subunits. Acquisition of novel molecular recognition features (MoRFs) through evolution of new subunits or through elongation of the existing subunits was evident in metazoans and plants. A new concept of ‘junction-MoRF’ has been introduced. Evolutionary link between CBP and Med15 has been provided which explain the evolution of extended-IDR in CBP from Med15 KIX-IDR junction-MoRF suggesting role of junction-MoRF in evolution and modulation of protein–protein interaction repertoire. This study can be informative and helpful in understanding the conserved and flexible nature of Mediator complex across eukaryotic kingdoms.
Department of Biotechnology, Government of India
[BT/PR14519/BRB/10/869/2010 and Innovative Young
Biotechnologist Award BT/BI/12/045/2008 to J.K.T.].
Funding for open access charge: From the institute.
Conflict of interest statement. None declared.
 
Date 2016-01-28T11:43:13Z
2016-01-28T11:43:13Z
2016
 
Type Article
 
Identifier Nucl. Acids Res., 44(4): 1591-1612
0305-1048
http://172.16.0.77:8080/jspui/handle/123456789/608
http://nar.oxfordjournals.org/content/early/2015/11/19/nar.gkv1135.abstract
10.1093/nar/gkv1135
 
Language en_US
 
Publisher Oxford University Press