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Advances in Agrobacterium tumefaciens-mediated genetic transformation of graminaceous crops

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Title Advances in Agrobacterium tumefaciens-mediated genetic transformation of graminaceous crops
 
Creator Singh, Roshan Kumar
Prasad, Manoj
 
Subject Agrobacterium tumefaciens
Genetic transformation
Cereal
Explant
Plant regeneration
 
Description Accepted date: 27 October 2015
Steady increase in global population poses several challenges to plant science research, including demand for increased crop productivity, grain yield, nutritional quality and improved tolerance to different environmental factors. Transgene-based approaches are promising to address these challenges by transferring potential candidate genes to host organisms through different strategies. Agrobacterium-mediated gene transfer is one such strategy which is well known for enabling efficient gene transfer in both monocot and dicots. Due to its versatility, this technique underwent several advancements including development of improved in vitro plant regeneration system, co-cultivation and selection methods, and use of hyper-virulent strains of Agrobacterium tumefaciens harbouring super-binary vectors. The efficiency of this method has also been enhanced by the use of acetosyringone to induce the activity of vir genes, silver nitrate to reduce the Agrobacterium-induced necrosis and cysteine to avoid callus browning during co-cultivation. In the last two decades, extensive efforts have been invested towards achieving efficient Agrobacterium-mediated transformation in cereals. Though high-efficiency transformation systems have been developed for rice and maize, comparatively lesser progress has been reported in other graminaceous crops. In this context, the present review discusses the progress made in Agrobacterium-mediated transformation system in rice, maize, wheat, barley, sorghum, sugarcane, Brachypodium, millets, bioenergy and forage and turf grasses. In addition, it also provides an overview of the genes that have been recently transferred to these graminaceous crops using Agrobacterium, bottlenecks in this technique and future possibilities for crop improvement.
The authors’ work in the area of millet genomics is
supported by the core grant of the National Institute of Plant Genome
Research (NIPGR), New Delhi, India. Mr. Roshan K Singh acknowledges the Council of Scientific and Industrial Research, Government of
India, New Delhi, for awarding Research Fellowship. The authors also
acknowledge Mr. Mehanathan Muthamilarasan, NIPGR, for his helpful
suggestions.
 
Date 2016-01-29T07:20:53Z
2016-01-29T07:20:53Z
2016
 
Type Article
 
Identifier Protoplasma, 253(3): 691-707
1615-6102
http://172.16.0.77:8080/jspui/handle/123456789/611
http://link.springer.com/article/10.1007%2Fs00709-015-0905-3
10.1007/s00709-015-0905-3
 
Language en_US
 
Publisher Springer