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Dehydration-responsive miRNAs in foxtail millet: genome-wide identification, characterization and expression profiling

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Title Dehydration-responsive miRNAs in foxtail millet: genome-wide identification, characterization and expression profiling
 
Creator Yadav, Amita
Khan, Yusuf
Prasad, Manoj
 
Subject Contrasting cultivars
Dehydration
Drought stress
miRNAs
Next generation sequencing
Post-transcriptional regulation
Small RNA library
Water deficit
 
Description Accepted date: 20 November 2015
MAIN CONCLUSION:

A set of novel and known dehydration-responsive miRNAs have been identified in foxtail millet. These findings provide new insights into understanding the functional role of miRNAs and their respective targets in regulating plant response to dehydration stress. MicroRNAs perform significant regulatory roles in growth, development and stress response of plants. Though the miRNA-mediated gene regulatory networks under dehydration stress remain largely unexplored in plant including foxtail millet (Setaria italica), which is a natural abiotic stress tolerant crop. To find out the dehydration-responsive miRNAs at the global level, four small RNA libraries were constructed from control and dehydration stress treated seedlings of two foxtail millet cultivars showing contrasting tolerance behavior towards dehydration stress. Using Illumina sequencing technology, 55 known and 136 novel miRNAs were identified, representing 22 and 48 miRNA families, respectively. Eighteen known and 33 novel miRNAs were differentially expressed during dehydration stress. After the stress treatment, 32 dehydration-responsive miRNAs were up-regulated in tolerant cultivar and 22 miRNAs were down-regulated in sensitive cultivar, suggesting that miRNA-mediated molecular regulation might play important roles in providing contrasting characteristics to these cultivars. Predicted targets of identified miRNAs were found to encode various transcription factors and functional enzymes, indicating their involvement in broad spectrum regulatory functions and biological processes. Further, differential expression patterns of seven known miRNAs were validated by northern blot and expression of ten novel dehydration-responsive miRNAs were confirmed by SL-qRT PCR. Differential expression behavior of five miRNA-target genes was verified under dehydration stress treatment and two of them also validated by RLM RACE. Overall, the present study highlights the importance of dehydration stress-associated post-transcriptional regulation governed by miRNAs and their targets in a naturally stress-tolerant model crop.
Authors’ work in the area of foxtail millet
genomics is supported by the Core Grant of National Institute of Plant
Genome Research, New Delhi, India. Ms. Amita Yadav acknowledges the Research Fellowship received from Council of Scientific
and Industrial Research (CSIR), New Delhi, India.
 
Date 2016-01-29T08:18:18Z
2016-01-29T08:18:18Z
2016
 
Type Article
 
Identifier Planta, 243(3): 749-766
1432-2048
http://172.16.0.77:8080/jspui/handle/123456789/612
http://link.springer.com/article/10.1007%2Fs00425-015-2437-7
10.1007/s00425-015-2437-7
 
Language en_US
 
Publisher Springer