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Fruit ripening regulation of α-Mannosidase expression by the MADS box transcription factor RIPENING INHIBITOR and ethylene

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Title Fruit ripening regulation of α-Mannosidase expression by the MADS box transcription factor RIPENING INHIBITOR and ethylene
 
Creator Irfan, Mohammad
Ghosh, Sumit
Meli, Vijaykumar S.
Kumar, Anil
Kumar, Vinay
Chakraborty, Niranjan
Chakraborty, Subhra
Datta, Asis
 
Subject fruit ripening
transcriptional regulation
fruit ripening-specific promoter
α-Man
RIN
 
Description Accepted date: 07 January 2016
α-Mannosidase (α-Man), a fruit ripening-specific N-glycan processing enzyme, is involved in ripening-associated fruit softening process. However, the regulation of fruit-ripening specific expression of α-Man is not well understood. We have identified and functionally characterized the promoter of tomato (Solanum lycopersicum) α-Man to provide molecular insights into its transcriptional regulation during fruit ripening. Fruit ripening-specific activation of the α-Man promoter was revealed by analysing promoter driven expression of beta-glucuronidase (GUS) reporter in transgenic tomato. We found that RIPENING INHIBITOR (RIN), a MADS box family transcription factor acts as positive transcriptional regulator of α-Man during fruit ripening. RIN directly bound to the α-Man promoter sequence and promoter activation/α-Man expression was compromised in rin mutant fruit. Deletion analysis revealed that a promoter fragment (567 bp upstream of translational start site) that contained three CArG boxes (binding sites for RIN) was sufficient to drive GUS expression in fruits. In addition, α-Man expression was down-regulated in fruits of Nr mutant which is impaired in ethylene perception and promoter activation/α-Man expression was induced in wild type following treatment with a precursor of ethylene biosynthesis, 1-aminocyclopropane-1-carboxylic acid (ACC). Although, α-Man expression was induced in rin mutant after ACC treatment, the transcript level was less as compared to ACC-treated wild type. Taken together, these results suggest RIN-mediated direct transcriptional regulation of α-Man during fruit ripening and ethylene may acts in RIN-dependent and -independent ways to regulate α-Man expression.
The present study was financially supported by a research grant (BT/01/CEIB/12/II/01) from the Department of Biotechnology and core research grant from National Institute of Plant Genome Research. MI thanks University Grant Commission for pre-doctoral fellowship.
 
Date 2016-02-09T05:32:18Z
2016-02-09T05:32:18Z
2016
 
Type Article
 
Identifier Front. Plant Sc., 7: 10
1664-462X
http://172.16.0.77:8080/jspui/handle/123456789/619
http://journal.frontiersin.org/article/10.3389/fpls.2016.00010/abstract
10.3389/fpls.2016.00010
 
Language en_US
 
Publisher Frontiers Media S.A.