Fruit ripening regulation of α-Mannosidase expression by the MADS box transcription factor RIPENING INHIBITOR and ethylene
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Title |
Fruit ripening regulation of α-Mannosidase expression by the MADS box transcription factor RIPENING INHIBITOR and ethylene
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Creator |
Irfan, Mohammad
Ghosh, Sumit Meli, Vijaykumar S. Kumar, Anil Kumar, Vinay Chakraborty, Niranjan Chakraborty, Subhra Datta, Asis |
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Subject |
fruit ripening
transcriptional regulation fruit ripening-specific promoter α-Man RIN |
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Description |
Accepted date: 07 January 2016
α-Mannosidase (α-Man), a fruit ripening-specific N-glycan processing enzyme, is involved in ripening-associated fruit softening process. However, the regulation of fruit-ripening specific expression of α-Man is not well understood. We have identified and functionally characterized the promoter of tomato (Solanum lycopersicum) α-Man to provide molecular insights into its transcriptional regulation during fruit ripening. Fruit ripening-specific activation of the α-Man promoter was revealed by analysing promoter driven expression of beta-glucuronidase (GUS) reporter in transgenic tomato. We found that RIPENING INHIBITOR (RIN), a MADS box family transcription factor acts as positive transcriptional regulator of α-Man during fruit ripening. RIN directly bound to the α-Man promoter sequence and promoter activation/α-Man expression was compromised in rin mutant fruit. Deletion analysis revealed that a promoter fragment (567 bp upstream of translational start site) that contained three CArG boxes (binding sites for RIN) was sufficient to drive GUS expression in fruits. In addition, α-Man expression was down-regulated in fruits of Nr mutant which is impaired in ethylene perception and promoter activation/α-Man expression was induced in wild type following treatment with a precursor of ethylene biosynthesis, 1-aminocyclopropane-1-carboxylic acid (ACC). Although, α-Man expression was induced in rin mutant after ACC treatment, the transcript level was less as compared to ACC-treated wild type. Taken together, these results suggest RIN-mediated direct transcriptional regulation of α-Man during fruit ripening and ethylene may acts in RIN-dependent and -independent ways to regulate α-Man expression. The present study was financially supported by a research grant (BT/01/CEIB/12/II/01) from the Department of Biotechnology and core research grant from National Institute of Plant Genome Research. MI thanks University Grant Commission for pre-doctoral fellowship. |
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Date |
2016-02-09T05:32:18Z
2016-02-09T05:32:18Z 2016 |
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Type |
Article
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Identifier |
Front. Plant Sc., 7: 10
1664-462X http://172.16.0.77:8080/jspui/handle/123456789/619 http://journal.frontiersin.org/article/10.3389/fpls.2016.00010/abstract 10.3389/fpls.2016.00010 |
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Language |
en_US
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Publisher |
Frontiers Media S.A.
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