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De novo transcriptome analysis of medicinally important Plantago ovata using RNA-seq

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Title De novo transcriptome analysis of medicinally important Plantago ovata using RNA-seq
 
Creator Kotwal, Shivanjali
Kaul, Sanjana
Sharma, Pooja
Gupta, Mehak
Shankar, Rama
Jain, Mukesh
Dhar, Manoj K.
 
Subject Plantago ovata
De novo assembly
RNA-Seq
 
Description Accepted date: February 11, 2016
Plantago ovata is an economically and medicinally important plant of the family Plantaginaceae. It is used extensively for the production of seed husk for its application in pharmaceutical, food and cosmetic industries. In the present study, the transcriptome of P. ovata ovary was sequenced using Illumina Genome Analyzer platform to characterize the mucilage biosynthesis pathway in the plant. De novo assembly was carried out using Oases followed by velvet. A total of 46,955 non-redundant transcripts (≥100 bp) using ~29 million high-quality paired end reads were generated. Functional categorization of these transcripts revealed the presence of several genes involved in various biological processes like metabolic pathways, mucilage biosynthesis, biosynthesis of secondary metabolites and antioxidants. In addition, simple sequence-repeat motifs, non-coding RNAs and transcription factors were also identified. Expression profiling of some genes involved in mucilage biosynthetic pathway was performed in different tissues of P. ovata using Real time PCR analysis. The study has resulted in a valuable resource for further studies on gene expression, genomics and functional genomics in P. ovata.
The authors are grateful to Department of Biotechnology (DBT), Govt. of India for financial assistance in the form of a research project to MKD. Ms. Shivanjali Kotwal is grateful to the Department of Science and Technology (DST), Govt. of India for an INSPIRE fellowship.
 
Date 2016-03-07T08:56:32Z
2016-03-07T08:56:32Z
2016
 
Type Article
 
Identifier PLoS One, 11(3): e0150273
1932-6203
http://172.16.0.77:8080/jspui/handle/123456789/627
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0150273#authcontrib
10.1371/journal.pone.0150273
 
Language en_US
 
Publisher PLOS