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Comparison of proteomic and metabolomic profiles of two contrasting ecotypes of sweetpotato (Ipomoea batata L)

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Title Comparison of proteomic and metabolomic profiles of two contrasting ecotypes of sweetpotato (Ipomoea batata L)
 
Creator Shekhar, Shubhendu
Mishra, Divya
Gayali, Saurabh
Buragohain, Alak Kumar
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Food security
Non-model crop
Sweetpotato
Ecotypes
Nutrient acquisition
Proteometabolomic
 
Description Accepted date: 16 March 2016
Sweetpotato has long been acknowledged as a significant contributor of global caloric needs, which continues to be of remarkable economic value. It is an important staple and emergency food in many countries and its annual world production hovers to about 130 million tons. The tubers act as sink and compete for the available photoassimilates eventually leading to the acquisition of nutrients and phytochemicals. Differential display of genes or gene-products, and metabolites causes differences in nutritive value of closely related ecotypes. To better understand the molecular basis for differential nutrient availability and phytochemicals, and exploit the natural genetic variation(s), we aimed at developing proteometabolic profiles of two contrasting ecotypes of sweetpotato. Proteomic analyses led to the identification of 1541 and 1201 proteins in orange fleshed and white fleshed sweetpotato ecotypes, respectively, presumably associated with binding, followed by catalytic, transferase, hydrolase, kinase and transporter activities. Furthermore, metabolome profiling revealed 148 and 126 metabolites in cv. OFSP and WFSP, respectively. This study would provide a basis for future comparative proteometabolomic efforts for sweetpotato, in particular and tuber crops in general. The results would expand our understanding of the proteome as well as metabolome and give new insights into how ecotype-specific traits are developed.
This work was supported by grants (BT/PR3123/16//250) from the Department of Biotechnology (DBT), Govt. of India. We kindly acknowledge the University Grant Commission (UGC) and CSIR, Govt. of India for providing predoctoral fellowship to D.M and S.G. We also thank CTCRI, India for providing planting materials of sweetpotato and JNU AIRF for GC-MS analysis. Assistance of Mr. Shankar Acharya and Mr. C. Ravishankar during field management is highly acknowledged.
 
Date 2016-03-23T05:59:51Z
2016-03-23T05:59:51Z
2016
 
Type Article
 
Identifier J. Proteomics, 143: 306-317
1874-3919
http://172.16.0.77:8080/jspui/handle/123456789/630
http://www.sciencedirect.com/science/article/pii/S1874391916300847
10.1016/j.jprot.2016.03.028
 
Language en_US
 
Publisher Elsevier B.V.