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WRKY domain-encoding genes of a crop legume chickpea (Cicer arietinum): comparative analysis with Medicago truncatula WRKY family and characterization of group-III gene(s)

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Title WRKY domain-encoding genes of a crop legume chickpea (Cicer arietinum): comparative analysis with Medicago truncatula WRKY family and characterization of group-III gene(s)
 
Creator Kumar, Kamal
Srivastava, Vikas
Purayannur, Savithri
Kaladhar, V. Chandra
Cheruvu, Purnima Jaiswal
Verma, Praveen K.
 
Subject Ascochyta rabiei
WRKY domain
Exo70
 
Description Accepted date: February 20, 2016
The WRKY genes have been identified as important transcriptional modulators predominantly during the environmental stresses, but they also play critical role at various stages of plant life cycle. We report the identification of WRKY domain (WD)-encoding genes from galegoid clade legumes chickpea (Cicer arietinum L.) and barrel medic (Medicago truncatula). In total, 78 and 98 WD-encoding genes were found in chickpea and barrel medic, respectively. Comparative analysis suggests the presence of both conserved and unique WRKYs, and expansion of WRKY family in M. truncatula primarily by tandem duplication. Exclusively found in galegoid legumes, CaWRKY16 and its orthologues encode for a novel protein having a transmembrane and partial Exo70 domains flanking a group-III WD. Genomic region of galegoids, having CaWRKY16, is more dynamic when compared with millettioids. In onion cells, fused CaWRKY16-EYFP showed punctate fluorescent signals in cytoplasm. The chickpea WRKY group-III genes were further characterized for their transcript level modulation during pathogenic stress and treatments of abscisic acid, jasmonic acid, and salicylic acid (SA) by real-time PCR. Differential regulation of genes was observed during Ascochyta rabiei infection and SA treatment. Characterization of A. rabiei and SA inducible gene CaWRKY50 showed that it localizes to plant nucleus, binds to W-box, and have a C-terminal transactivation domain. Overexpression of CaWRKY50 in tobacco plants resulted in early flowering and senescence. The in-depth comparative account presented here for two legume WRKY genes will be of great utility in hastening functional characterization of crop legume WRKYs and will also help in characterization of Exo70Js.
This work was funded by Department of Biotechnology, Government of India through research grant for the Challenge Program on Chickpea Functional Genomics project (Sanction No. BT/AGR/CG-Phase II/01/2014) and core grant from National Institute of Plant Genome Research, New Delhi, India. Funding to pay the Open Access publication charges for this article was provided by the National Institute of Plant Genome Research, New Delhi, India.
 
Date 2016-04-18T09:07:51Z
2016-04-18T09:07:51Z
2016
 
Type Article
 
Identifier DNA Res., 23(3): 225-239
1756-1663
http://172.16.0.77:8080/jspui/handle/123456789/636
http://dnaresearch.oxfordjournals.org/content/early/2016/04/08/dnares.dsw010.short?rss=1
10.1093/dnares/dsw010
 
Language en_US
 
Publisher Oxford University Press