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The α-crystallin domain containing genes: identification, phylogeny and expression profiling in abiotic stress, phytohormone response and development in tomato (Solanum lycopersicum)

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Title The α-crystallin domain containing genes: identification, phylogeny and expression profiling in abiotic stress, phytohormone response and development in tomato (Solanum lycopersicum)
 
Creator Paul, Asosii
Rao, Sombir
Mathur, Saloni
 
Subject Solanum lycopersicum
abiotic stress
gene expression
sHSPs
α-crystallin domain
 
Description Accepted date: 18 March 2016
The α-crystallin domain (ACD) is an ancient domain conserved among all kingdoms. Plant ACD proteins have roles in abiotic stresses, transcriptional regulation, inhibiting virus movement, and DNA demethylation. An exhaustive in-silico analysis using Hidden Markov Model-based conserved motif search of the tomato proteome yielded a total of 50 ACD proteins that belonged to four groups, sub-divided further into 18 classes. One of these groups belongs to the small heat shock protein (sHSP) class of proteins, molecular chaperones implicated in heat tolerance. Both tandem and segmental duplication events appear to have shaped the expansion of this gene family with purifying selection being the primary driving force for evolution. The expression profiling of the Acd genes in two different heat stress regimes suggested that their transcripts are differentially regulated with roles in acclimation and adaptive response during recovery. The co-expression of various genes in response to different abiotic stresses (heat, low temperature, dehydration, salinity, and oxidative stress) and phytohormones (abscisic acid and salicylic acid) suggested possible cross-talk between various members to combat a myriad of stresses. Further, several genes were highly expressed in fruit, root, and flower tissues as compared to leaf signifying their importance in plant development too. Evaluation of the expression of this gene family in field grown tissues highlighted the prominent role they have in providing thermo-tolerance during daily temperature variations. The function of three putative sHSPs was established as holdase chaperones as evidenced by protection to malate-dehydrogenase against heat induced protein-aggregation. This study provides insights into the characterization of the Acd genes in tomato and forms the basis for further functional validation in-planta.
Authors thank the Central Instrumentation Facility, NIPGR for qPCR and the Phytotron facility at NIPGR for growing plants and stress treatments. SR acknowledges DBT for the award of Junior Research Fellowship. SM acknowledges the financial grant as part of SERB Women Excellence Award from the Department of Science and Technology, Government of India.
 
Date 2016-04-18T09:29:09Z
2016-04-18T09:29:09Z
2016
 
Type Article
 
Identifier Front. Plant Sc., 7: 426
1664-462X
http://172.16.0.77:8080/jspui/handle/123456789/637
http://journal.frontiersin.org/article/10.3389/fpls.2016.00426/full
10.3389/fpls.2016.00426
 
Language en_US
 
Publisher Frontiers Media S.A.