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A positive feedback loop governed by SUB1A1 interaction with Mitogen Activated Protein Kinase 3 imparts submergence tolerance in rice

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Title A positive feedback loop governed by SUB1A1 interaction with Mitogen Activated Protein Kinase 3 imparts submergence tolerance in rice
 
Creator Singh, Pallavi
Sinha, Alok Krishna
 
Subject MAPK
Oryza sativa
submergence tolerance
Rice
 
Description Accepted date: April 12, 2016
Mitogen-activated protein kinase (MAPK) signal transduction networks have been extensively explored in plants; however, the connection between MAPK signalling cascades and submergence tolerance is currently unknown. The ethylene response factor-like protein SUB1A orchestrates a plethora of responses during submergence stress tolerance in rice. In the present study, we report that MPK3 is activated by submergence in a SUB1A-dependent manner. MPK3 physically interacts with and phosphorylates SUB1A in a tolerant-allele-specific manner. Furthermore, the tolerant allele SUB1A1 binds to the MPK3 promoter and regulates its expression in a positive regulatory loop during submergence stress signalling. We present molecular and physiological evidence for the key role of the MPK3-SUB1A1 module in acclimation of rice seedlings to the adverse effects of submergence. Overall, the results provide a mechanistic understanding of submergence tolerance in Oryza sativa.
The work was supported by the core grant of National Institute of Plant Genome Research from the Department of Biotechnology, Government of India. P.S. is a recipient of a fellowship from the Council of Scientific and Industrial Research, Government of India. The authors also thank the Confocal Microscopy Facility and the Central Instrumentation Facility of NIPGR, New Delhi, India. The authors acknowledge Prof. S. Robin from Tamil Nadu Agricultural University for providing us with the seeds of Swarna and Swarna Sub1. Authors thank Prof. Indranil Das Gupta, University of Delhi South Campus, New Delhi for the kind gift of mVIGS vector. Authors thank Prof. Adam J. Bogdanove, Cornell University, Ithaca for critically reading and editing the manuscript.
 
Date 2016-04-18T10:02:04Z
2016-04-18T10:02:04Z
2016
 
Type Article
 
Identifier Plant Cell, 28(5): 1127-1143
1532-298X
http://172.16.0.77:8080/jspui/handle/123456789/639
http://www.plantcell.org/content/early/2016/04/14/tpc.15.01001.abstract
http:/​/​dx.​doi.​org/​10.​1105/​tpc.​15.​01001
 
Language en_US
 
Publisher American Society of Plant Biologists