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Draft genome sequencing and secretome analysis of fungal phytopathogen Ascochyta rabiei provides insight into the necrotrophic effector repertoire

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Title Draft genome sequencing and secretome analysis of fungal phytopathogen Ascochyta rabiei provides insight into the necrotrophic effector repertoire
 
Creator Verma, Sandhya
Gazara, Rajesh Kumar
Nizam, Shadab
Parween, Sabiha
Chattopadhyay, Debasis
Verma, Praveen K.
 
Subject Effectors in plant pathology
Fungal genomics
 
Description Accepted date: 04 April 2016
Constant evolutionary pressure acting on pathogens refines their molecular strategies to attain successful pathogenesis. Recent studies have shown that pathogenicity mechanisms of necrotrophic fungi are far more intricate than earlier evaluated. However, only a few studies have explored necrotrophic fungal pathogens. Ascochyta rabiei is a necrotrophic fungus that causes devastating blight disease of chickpea (Cicer arietinum). Here, we report a 34.6 megabase draft genome assembly of A. rabiei. The genome assembly covered more than 99% of the gene space and 4,259 simple sequence repeats were identified in the assembly. A total of 10,596 high confidence protein-coding genes were predicted which includes a large and diverse inventory of secretory proteins, transporters and primary and secondary metabolism enzymes reflecting the necrotrophic lifestyle of A. rabiei. A wide range of genes encoding carbohydrate-active enzymes capable for degradation of complex polysaccharides were also identified. Comprehensive analysis predicted a set of 758 secretory proteins including both classical and non-classical secreted proteins. Several of these predicted secretory proteins showed high cysteine content and numerous tandem repeats. Together, our analyses would broadly expand our knowledge and offer insights into the pathogenesis and necrotrophic lifestyle of fungal phytopathogens.
We gratefully acknowledge research grant from Department of Biotechnology, Government of India (File No: BT/PR7164/PBD/1016/2012) and core grant from National Institute of Plant Genome Research, New Delhi, India, for funding this work. We are also thankful to the Director, National Institute of Plant Genome Research for providing facilities and help during this study. We thank Centre for Cellular and Molecular Platforms (C-CAMP), Bangalore, India for the performing the genome sequencing.
 
Date 2016-04-21T08:50:57Z
2016-04-21T08:50:57Z
2016
 
Type Article
 
Identifier Scientific Reports, 6: 24638
2045-2322
http://172.16.0.77:8080/jspui/handle/123456789/640
http://www.nature.com/articles/srep24638
10.1038/srep24638
 
Language en_US
 
Publisher Nature Publishing Group