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Localization of nitric oxide in wheat roots by DAF fluorescence

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Title Localization of nitric oxide in wheat roots by DAF fluorescence
 
Creator Wany, Aakanksha
Gupta, Kapuganti Jagadis
 
Subject DAF fluorescence
Hypoxia
Microscope
Microtome
Nitric oxide
 
Description Accepted date: 20 April 2016
Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofluorescein in diacetate form (DAF-2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofluorescein diacetate (DAF-2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofluorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofluorescein). There are various advantages and disadvantages associated with this method, but to its advantage in diffusion closely to NO producing sites, it is widely used for localization studies. Here, we describe method to make sections of the roots and localization of NO in roots subjected to hypoxic stress.
 
Date 2016-04-22T05:57:07Z
2016-04-22T05:57:07Z
2016
 
Type Book Chapter
 
Identifier Methods Mol. Biol., 1424: 39-47
978-1-4939-3600-7
http://172.16.0.77:8080/jspui/handle/123456789/641
http://link.springer.com/protocol/10.1007%2F978-1-4939-3600-7_4
10.1007/978-1-4939-3600-7_4
 
Language en_US
 
Publisher Springer