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Nitric oxide measurement from purified enzymes and estimation of scavenging activity by gas phase chemiluminescence method

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Title Nitric oxide measurement from purified enzymes and estimation of scavenging activity by gas phase chemiluminescence method
 
Creator Kumari, Aprajita
Gupta, Alok Kumar
Mishra, Sonal
Wany, Aakanksha
Gupta, Kapuganti Jagadis
 
Subject Chemiluminescence
Mitochondria
Nitrate reductase
Nitric oxide synthase
Scavenging
 
Description Accepted date: 20 April 2016
In plants, nitrate reductase (NR) is a key enzyme that produces nitric oxide (NO) using nitrite as a substrate. Lower plants such as algae are shown to have nitric oxide synthase enzyme and higher plants contain NOS activity but enzyme responsible for NO production in higher plants is subjected to debate. In plant nitric oxide research, it is very important to measure NO very precisely in order to determine its functional role. A significant amount of NO is being scavenged by various cell components. The net NO production depends in production minus scavenging. Here, we describe methods to measure NO from purified NR and inducible nitric oxide synthase from mouse (iNOS), we also describe a method of measure NO scavenging by tobacco cell suspensions and mitochondria from roots.
 
Date 2016-04-22T06:05:33Z
2016-04-22T06:05:33Z
2016
 
Type Book chapter
 
Identifier Methods Mol. Biol., 1424: 31-38
978-1-4939-3600-7
http://172.16.0.77:8080/jspui/handle/123456789/642
http://link.springer.com/protocol/10.1007%2F978-1-4939-3600-7_3
10.1007/978-1-4939-3600-7_3
 
Language en_US
 
Publisher Springer