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Chemiluminescence detection of nitric oxide from roots, leaves, and root mitochondria

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Title Chemiluminescence detection of nitric oxide from roots, leaves, and root mitochondria
 
Creator Wany, Aakanksha
Gupta, Alok Kumar
Kumari, Aprajita
Gupta, Shika
Mishra, Sonal
Jaintu, Ritika
Pathak, Pradeep K.
Gupta, Kapuganti Jagadis
 
Subject Cell suspensions
Chemiluminescence
Hydroponics
Mitochondria
Nitric oxide
 
Description Accepted date: 20 April 2016
NO is a free radical with short half-life and high reactivity; due to its physiochemical properties it is very difficult to detect the concentrations precisely. Chemiluminescence is one of the robust methods to quantify NO. Detection of NO by this method is based on reaction of nitric oxide with ozone which leads to emission of light and amount of light is proportional to NO. By this method NO can be measured in the range of pico moles to nano moles range. Using direct chemiluminescence method, NO emitted into the gas stream can be detected whereas using indirect chemiluminescence oxidized forms of NO can also be detected. We detected NO using purified nitrate reductase, mitochondria, cell suspensions, and roots; detail measurement method is described here.
 
Date 2016-04-22T06:31:07Z
2016-04-22T06:31:07Z
2016
 
Type Book chapter
 
Identifier Methods Mol. Biol., 1424: 15-29
978-1-4939-3600-7
http://172.16.0.77:8080/jspui/handle/123456789/643
http://link.springer.com/protocol/10.1007%2F978-1-4939-3600-7_2
10.1007/978-1-4939-3600-7_2
 
Language en_US
 
Publisher Springer