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Rice and chickpea GDPDs are preferentially influenced by low phosphate and CaGDPD1 encodes an active glycerophosphodiester phosphodiesterase enzyme

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Title Rice and chickpea GDPDs are preferentially influenced by low phosphate and CaGDPD1 encodes an active glycerophosphodiester phosphodiesterase enzyme
 
Creator Mehra, P.
Giri, Jitender
 
Subject Glycerophosphodiester phosphodiesterase
Nutrient deficiencies
Phospholipid signaling
Phospholipid degradation
Expression profiling
 
Description Accepted date: 13 April 2016
Rice and chickpea GDPD s are transcriptionally influenced by mineral deficiencies; especially, by phosphate starvation and CaGDP1 encodes an active glycerophosphodiester phosphodiesterase enzyme. Glycerophosphodiester phosphodiesterases (GDPDs) are enzymes involved in the degradation of glycerophosphodiesters into sn-glycerol-3-phosphate and corresponding alcohols. These phospholipid remodeling genes have been suggested to play important roles in phosphate homeostasis. However, comprehensive information about the role of GDPDs under low phosphate (P) and other nutrient deficiencies (N, K, Fe, Zn) in rice and chickpea is missing. Here, we identified 13 OsGDPDs and 6 CaGDPDs in rice and chickpea, respectively, and partly characterized their roles in multiple nutrient stresses. Expression profiling after 7 and 15 days of deficiency treatments revealed unique and overlapping differential expression patterns of OsGDPDs and CaGDPDs under different nutrient stresses. Principal component analysis on the expression patterns of OsGDPDs and CaGDPDs revealed their preferential role in P starvation. Some of the GDPDs were also induced by N, K, Fe and Zn deficiency in temporal manner in both crops suggesting their roles in multiple nutrient stresses. Biochemical characterization of highly responsive chickpea GDPD, CaGDPD1, confirmed its in vitro GDPD activity and revealed its optimal temperature, pH and cofactor requirements. Further, CaGDPD1 showed its accumulation in ER and endomembranes. We hereby propose CaGDPD1 and various OsGDPDs as low P responsive marker genes in chickpea and rice, respectively. Our data uphold role of GDPDs in multinutrient responses and suggest them as candidates for rice and chickpea improvement for tolerance to various nutrient deficiencies.
This work was supported by the research Grant
of DBT (Grant No. BT/PR3299/AGR/2/813/2011), Government of
India. P.M. acknowledge the financial support from CSIR, India.
 
Date 2016-05-03T07:16:14Z
2016-05-03T07:16:14Z
2016
 
Type Article
 
Identifier Plant Cell Reports, 35(8): 1699-1717
1432-203X
http://172.16.0.77:8080/jspui/handle/123456789/645
http://link.springer.com/article/10.1007%2Fs00299-016-1984-0
10.1007/s00299-016-1984-0
 
Language en_US
 
Publisher Springer