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Ectopic expression of amaranth seed storage albumin modulates photoassimilate transport and nutrient acquisition in sweetpotato

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Title Ectopic expression of amaranth seed storage albumin modulates photoassimilate transport and nutrient acquisition in sweetpotato
 
Creator Shekhar, Shubhendu
Agrawal, Lalit
Mishra, Divya
Buragohain, Alak Kumar
Unnikrishnan, Mullath
Mohan, Chokkappan
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Plant molecular biology
Plant biotechnology
Molecular engineering in plants
 
Description Accepted date: 18 April 2016
Storage proteins in plants, because of high nutrient value, have been a subject of intensive investigation. These proteins are synthesized de novo in the cytoplasm and transported to the storage organelles where they serve as reservoir of energy and supplement of nitrogen during rapid growth and development. Sweetpotato is the seventh most important food crop worldwide, and has a significant contribution to the source of nutrition, albeit with low protein content. To determine the behaviour of seed storage proteins in non-native system, a seed albumin, AmA1, was overexpressed in sweetpotato with an additional aim of improving nutritional quality of tuber proteins. Introduction of AmA1 imparted an increase in protein and amino acid contents as well as the phytophenols. The proteometabolomics analysis revealed a rebalancing of the proteome, with no significant effects on the global metabolome profile of the transgenic tubers. Additionally, the slower degradation of starch and cellulose in transgenic tubers, led to increased post-harvest durability. Present study provides a new insight into the role of a seed storage protein in the modulation of photoassimilate movement and nutrient acquisition.
This work was supported by grants (BT/PR3123/16//250) from the Department of Biotechnology (DBT), Govt. of India. We kindly acknowledge the University Grant Commission (UGC), Govt. of India for providing predoctoral fellowship to D.M. We also thank CTCRI, India for providing planting materials of sweetpotato. We thank Department of Food Engineering and Technology, Department of Physics and Department of Chemistry, Tezpur University, and AIRF, JNU for extending their facilities. Assistance of Mr. Jasbeer Singh for illustrations and graphical representations, and Mr. Shankar Acharya and Mr. C. Ravishankar during field and growth room management is highly acknowledged.
 
Date 2016-05-09T10:27:13Z
2016-05-09T10:27:13Z
2016
 
Type Article
 
Identifier Scientific Reports, 6: 25384
2045-2322
http://172.16.0.77:8080/jspui/handle/123456789/650
http://www.nature.com/articles/srep25384
10.1038/srep25384
 
Language en_US
 
Publisher Nature Publishing Group