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Proteometabolomic analysis of transgenic tomato overexpressing oxalate decarboxylase uncovers novel proteins potentially involved in defense mechanism against Sclerotinia

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Title Proteometabolomic analysis of transgenic tomato overexpressing oxalate decarboxylase uncovers novel proteins potentially involved in defense mechanism against Sclerotinia
 
Creator Ghosh, Sudip
Narula, Kanika
Sinha, Arunima
Ghosh, Rajgourab
Jawa, Priyanka
Chakraborty, Niranjan
Chakraborty, Subhra
 
Subject Oxalic acid
Comparative proteomics and protein network
Immunity
Metabolomics
Oxalate decarboxylase
Tomato
 
Description Accepted date: 28 April 2016
Oxalic acid (OA) plays dual role in fungal pathogenicity in a concentration dependent manner. While at higher concentration it induces programmed cell death leading to fungal invasion, low oxalate build resistance in plant. Although OA has been identified as a virulence determinant for rot disease caused by Sclerotinia sp., our understanding of how oxalate downregulation impart host immunity is limited. We have earlier shown that ectopic expression of oxalate decarboxylase (FvOXDC) specifically degrades OA in tomato (Solanum lycopersicum). To elucidate low oxalate regulated molecular mechanism imparting immunity, a comparative proteomics approach has been applied to E8.2-OXDC tomato fruit displaying fungal resistance. Mass spectrometric analyses identified 92 OXDC-responsive immunity related protein spots (ORIRPs) presumably associated with acid metabolism, defense signaling and endoplasmic reticulum stress. Metabolome study indicated increased abundance of some of the organic acids paralleling the proteomic analysis. Further, we interrogated the proteome data using network analysis that identified modules enriched in known and novel immunity-related prognostic proteins centered around 14-3-3, translationally controlled tumor protein, annexin and chaperonin. Taken together, our data demonstrate that low oxalate may act as metabolic and immunity determinant through translational reprogramming.
This work was supported by grants from the National Institute of Plant Genome Research, New Delhi, India to S.C. K.N. is the recipient of post-doctoral fellowship from the Department of Biotechnology (DBT), Govt. of India. R.G. and A.S. are the recipients of pre-doctoral fellowship from the Council of Scientific and Industrial research (CSIR), Govt. of India. P.J. is the recipient of pre-doctoral fellowship from the University Grants Commission (UGC), Govt. of India. Authors also thank Mr. Jasbeer Singh for illustrations and graphical representations in the manuscript.
 
Date 2016-05-13T04:15:19Z
2016-05-13T04:15:19Z
2016
 
Type Article
 
Identifier J. Proteomics, 143: 242-253
1874-3919
http://172.16.0.77:8080/jspui/handle/123456789/651
http://www.sciencedirect.com/science/article/pii/S1874391916301804
10.1016/j.jprot.2016.04.047
 
Language en_US
 
Publisher Elsevier B.V.