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Molecular cloning and expression profiling of multiple Dof genes of Sorghum bicolor (L) Moench

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Title Molecular cloning and expression profiling of multiple Dof genes of Sorghum bicolor (L) Moench
 
Creator Gupta, Shubhra
Arya, Gulab C.
Malviya, Neha
Bisht, Naveen C.
Yadav, Dinesh
 
Subject DNA binding with one finger (Dof)
Sorghum
Expression profiling
Abiotic stress
Transcription factor
 
Description Accepted date: 20 May 2016
DNA binding with one finger (Dof) proteins represent a family of plant specific transcription factors associated with diverse biological processes, such as seed maturation and germination, phytohormone and light mediated regulation, and plant responses to biotic and abiotic stresses. In present study, a total of 21 Dof genes from Sorghum bicolor were cloned, sequenced and in silico characterized for homology search, revealing their identity to Dof like proteins. The expression profiling of SbDof genes using quantitative RT-PCR in different tissue types and also under drought and salt stresses was attempted. The SbDof genes displayed differential expression either in their transcript abundance or in their expression patterns under normal growth condition. Two of the SbDof genes namely SbDof8 and SbDof12 showed comparatively high level of transcript abundance in all the tissue types tested; whereas some of the SbDof genes showed a distinct tissue specific expression pattern. Further a total of 13 SbDof genes showed differential expression when subjected to either of the abiotic stress i.e. drought or salinity. Three of the SbDof genes namely SbDof12, SbDof19 and SbDof24 were found to be up-regulated in response to drought and salt stress. Comparative analysis of SbDof genes expression revealed existence of a complex transcriptional and functional diversity across plant growth and developmental stages.
The authors acknowledge Head, Department of
Biotechnology for providing infrastructural facilities. Prof. A. K.
Tyagi, Director National Institute for Plant Genome Research, New
Delhi is thankfully acknowledged for allowing SG to perform gene
expression work. SG and GCA acknowledge Council of Scientific and Industrial Research for Senior Research fellowship. The author
wishes to acknowledge Prof. P.K Shrotria, Department of Genetics
and Plant Breeding, College of Agriculture, G.B. Pant University of
Agriculture and Technology, Pantnagar, Uttarakhand for providing
the seeds of sorghum.
 
Date 2016-05-30T06:42:02Z
2016-05-30T06:42:02Z
2016
 
Type Article
 
Identifier Mol. Biol. Rep., 43(8): 767-774
1573-4978
http://172.16.0.77:8080/jspui/handle/123456789/654
http://link.springer.com/article/10.1007%2Fs11033-016-4019-6
10.1007/s11033-016-4019-6
 
Language en_US
 
Publisher Springer