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Tomato 26S Proteasome subunit RPT4a regulates ToLCNDV transcription and activates hypersensitive response in tomato

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Title Tomato 26S Proteasome subunit RPT4a regulates ToLCNDV transcription and activates hypersensitive response in tomato
 
Creator Sahu, Pranav Pankaj
Sharma, Namisha
Puranik, Swati
Chakraborty, Supriya
Prasad, Manoj
 
Subject Biotic
Plant molecular biology
 
Description Accepted date: 09 May 2016
Involvement of 26S proteasomal subunits in plant pathogen-interactions, and the roles of each subunit in independently modulating the activity of many intra- and inter-cellular regulators controlling physiological and defense responses of a plant were well reported. In this regard, we aimed to functionally characterize a Solanum lycopersicum 26S proteasomal subunit RPT4a (SlRPT4) gene, which was differentially expressed after Tomato leaf curl New Delhi virus (ToLCNDV) infection in tolerant cultivar H-88-78-1. Molecular analysis revealed that SlRPT4 protein has an active ATPase activity. SlRPT4 could specifically bind to the stem-loop structure of intergenic region (IR), present in both DNA-A and DNA-B molecule of the bipartite viral genome. Lack of secondary structure in replication-associated gene fragment prevented formation of DNA-protein complex suggesting that binding of SlRPT4 with DNA is secondary structure specific. Interestingly, binding of SlRPT4 to IR inhibited the function of RNA Pol-II and subsequently reduced the bi-directional transcription of ToLCNDV genome. Virus-induced gene silencing of SlRPT4 gene incited conversion of tolerant attributes of cultivar H-88-78-1 into susceptibility. Furthermore, transient overexpression of SlRPT4 resulted in activation of programmed cell death and antioxidant enzymes system. Overall, present study highlights non-proteolytic function of SlRPT4 and their participation in defense pathway against virus infection in tomato.
We are grateful to the Director, National Institute of Plant Genome Research (NIPGR) and Dean, School of Life Sciences, Jawaharlal Nehru University for providing facilities. We gratefully acknowledge the financial support from Department of Biotechnology (Grant No. BT/PR8357/PBD/16/1033/2013), Govt. of India and core grant of NIPGR, New Delhi, India. Authors also acknowledge Prof. Savithramma Dinesh-Kumar, Plant Biology Department, University of California, Davis, for providing TRV-VIGS vectors.
 
Date 2016-06-06T10:24:04Z
2016-06-06T10:24:04Z
2016
 
Type Article
 
Identifier Scientific Reports, 6: 27078
2045-2322
http://172.16.0.77:8080/jspui/handle/123456789/657
http://www.nature.com/articles/srep27078
10.1038/srep27078
 
Language en_US
 
Publisher Nature Publishing Group