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Transcriptome analysis of sunflower genotypes with contrasting oxidative stress tolerance reveals individual- and combined- biotic and abiotic stress tolerance mechanisms

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Title Transcriptome analysis of sunflower genotypes with contrasting oxidative stress tolerance reveals individual- and combined- biotic and abiotic stress tolerance mechanisms
 
Creator Ramu, Vemanna S.
Paramanantham, Anjugam
Ramegowda, Venkategowda
Mohan-Raju, Basavaiah
Udayakumar, Makarla
Senthil-Kumar, Muthappa
 
Subject Biotic and abiotic stress tolerance
Transcriptome Analysis
Sunflower Genotypes
 
Description Accepted date: June 1, 2016
In nature plants are often simultaneously challenged by different biotic and abiotic stresses. Although the mechanisms underlying plant responses against single stress have been studied considerably, plant tolerance mechanisms under combined stress is not understood. Also, the mechanism used to combat independently and sequentially occurring many number of biotic and abiotic stresses has also not systematically studied. From this context, in this study, we attempted to explore the shared response of sunflower plants to many independent stresses by using meta-analysis of publically available transcriptome data and transcript profiling by quantitative PCR. Further, we have also analyzed the possible role of the genes so identified in contributing to combined stress tolerance. Meta-analysis of transcriptomic data from many abiotic and biotic stresses indicated the common representation of oxidative stress responsive genes. Further, menadione-mediated oxidative stress in sunflower seedlings showed similar pattern of changes in the oxidative stress related genes. Based on this a large scale screening of 55 sunflower genotypes was performed under menadione stress and those contrasting in oxidative stress tolerance were identified. Further to confirm the role of genes identified in individual and combined stress tolerance the contrasting genotypes were individually and simultaneously challenged with few abiotic and biotic stresses. The tolerant hybrid showed reduced levels of stress damage both under combined stress and few independent stresses. Transcript profiling of the genes identified from meta-analysis in the tolerant hybrid also indicated that the selected genes were up-regulated under individual and combined stresses. Our results indicate that menadione-based screening can identify genotypes not only tolerant to multiple number of individual biotic and abiotic stresses, but also the combined stresses.
Combined stress tolerance projects at MS-K lab are supported by National Institute of Plant Genome Research (NIPGR) core funding and DBT-Ramalingaswami re-entry fellowship grant (BT/RLF/re-entry/23/2012). MUK lab is supported by Indian Council of Agricultural Research niche area of excellence program (F. No. 10-(6)/2005 EPD & F.No.10 (15) 2012 EPD). VSR is currently Fulbright-Nehru postdoctoral fellow under United States-India Educational Foundation (USIEF). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
 
Date 2016-06-21T11:37:37Z
2016-06-21T11:37:37Z
2016
 
Type Article
 
Identifier PLoS One, 11(6): e0157522
1932-6203
http://172.16.0.77:8080/jspui/handle/123456789/660
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0157522
10.1371/journal.pone.0157522
 
Language en_US
 
Publisher PLOS