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Expression of AtMed15 of Arabidopsis in yeast causes flocculation and increases ethanol production in yeast culture

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Title Expression of AtMed15 of Arabidopsis in yeast causes flocculation and increases ethanol production in yeast culture
 
Creator Dahiya, Pradeep
Bhat, Divya S.
Thakur, Jitendra K.
 
Subject Applied microbiology
Industrial microbiology
 
Description Accepted date: 27 May 2016
Mediator, a multiprotein complex involved in transcription of class II genes, was first discovered in yeast and then characterized in many metazoans revealing a striking structural conservation of the complex. However, sequences of Mediator subunits are not well conserved raising a question on the functional conservation of these individual subunits. In this study, expression of Med15 of Arabidopsis (AtMed15) in gal11∆ yeast could not complement the function of ScGal11 in galactose metabolism and resistance against cycloheximide. Surprisingly, AtMed15 changed the morphology of the yeast cells. The cells adhered strongly on the surface of the agar media, and showed robust flocculation in the liquid media without affecting the growth. The AtMed15-induced adhesion and flocculation were observed in different carbon sources. Calcium-assisted cell wall-bound mannan-binding proteins were found to be involved in this flocculation, which was unaffected by wide fluctuation of pH or temperatures revealing its constitutive robust nature. Expression of few flocculation related Flo genes was up-regulated in these cells. Interestingly, there was significant increase in ethanol production by the yeast expressing AtMed15. Robust and constitutive flocculation and increased ethanol production by yeast cells harbouring AtMed15 indicate an opportunity of its important usage in biotechnology industries.
This work was supported by IYBA grant (BT/BI/12/045/2008) to J.K.T. from the Department of Biotechnology, Ministry of Science and Technology, Government of India. Fellowship from DBT to P.D. and Short Term Research Fellowship from NIPGR to D.S.B. are acknowledged. We thank Dr. Atul Narang and Ms Shradha Mitra (both from Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, New Delhi) for their help with fermentor and estimation of ethanol concentration. We thank Central Instrumentation Facility of NIPGR for support in several experiments.
 
Date 2016-06-23T10:39:29Z
2016-06-23T10:39:29Z
2016
 
Type Article
 
Identifier Scientific Reports, 6: 27967
2045-2322
http://172.16.0.77:8080/jspui/handle/123456789/661
http://www.nature.com/articles/srep27967
10.1038/srep27967
 
Language en_US
 
Publisher Nature Publishing Group