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Identification of novel miRNAs from drought tolerant rice variety Nagina 22

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Title Identification of novel miRNAs from drought tolerant rice variety Nagina 22
 
Creator Mutum, Roseeta Devi
Kumar, Santosh
Balyan, Sonia
Kansal, Shivani
Mathur, Saloni
Raghuvanshi, Saurabh
 
Subject Plant evolution
Plant molecular biology
 
Description Accepted date: 11 July 2016
MicroRNAs regulate a spectrum of developmental and biochemical processes in plants and animals. Thus, knowledge of the entire miRNome is essential to understand the complete regulatory schema of any organism. The current study attempts to unravel yet undiscovered miRNA genes in rice. Analysis of small RNA libraries from various tissues of drought-tolerant 'aus' rice variety Nagina 22 (N22) identified 71 novel miRNAs. These were validated based on precursor hairpin structure, small RNA mapping pattern, 'star' sequence, conservation and identification of targets based on degradome data. While some novel miRNAs were conserved in other monocots and dicots, most appear to be lineage-specific. They were segregated into two different classes based on the closeness to the classical miRNA definition. Interestingly, evidence of a miRNA-like cleavage was found even for miRNAs that lie beyond the classical definition. Several novel miRNAs displayed tissue-enriched and/or drought responsive expression. Generation and analysis of the degradome data from N22 along with publicly available degradome identified several high confidence targets implicated in regulation of fundamental processes such as flowering and stress response. Thus, discovery of these novel miRNAs considerably expands the dimension of the miRNA-mediated regulation in rice.
This work was supported by the Department of Biotechnology (DBT), Government of India (Grant no. BT/PR10071/AGR/36/31/2007 and BT/PR628/AGR/36/674/2011). R.D.M. acknowledges fellowships from UGC (University Grants Commission) and University of Delhi, S. Kumar from DBT, S.B. from University of Delhi, S. Kansal from CSIR (Council of Scientific and Industrial Research), India.
 
Date 2016-08-10T06:40:18Z
2016-08-10T06:40:18Z
2016
 
Type Article
 
Identifier Scientific Reports, 6: 30786
2045-2322
http://172.16.0.77:8080/jspui/handle/123456789/671
http://www.nature.com/articles/srep30786
10.1038/srep30786
 
Language en_US
 
Publisher Nature Publishing Group