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Proteometabolomic study of compatible interaction in tomato fruit challenged with Sclerotinia rolfsii illustrates novel protein network during disease progression

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Title Proteometabolomic study of compatible interaction in tomato fruit challenged with Sclerotinia rolfsii illustrates novel protein network during disease progression
 
Creator Ghosh, Sudip
Narula, Kanika
Sinha, Arunima
Ghosh, Rajgourab
Jawa, Priyanka
Chakraborty, Niranjan
Chakraborty, Subhra
 
Subject Sclerotinia rolfsii
Proteometabolomic
tomato fruit
protein network
patho-stress
2-DE coupled mass spectrometry
comparative proteomics
metabolite profiling
 
Description Accepted date: 30 June 2016
Fruit is an assimilator of metabolites, nutrients, and signaling molecules, thus considered as potential target for pathogen attack. In response to patho-stress, such as fungal invasion, plants reorganize their proteome, and reconfigure their physiology in the infected organ. This remodeling is coordinated by a poorly understood signal transduction network, hormonal cascades, and metabolite reallocation. The aim of the study was to explore organ-based proteomic alterations in the susceptibility of heterotrophic fruit to necrotrophic fungal attack. We conducted time-series protein profiling of Sclerotinia rolfsii invaded tomato (Solanum lycopersicum) fruit. The differential display of proteome revealed 216 patho-stress responsive proteins (PSRPs) that change their abundance by more than 2.5-fold. Mass spectrometric analyses led to the identification of 56 PSRPs presumably involved in disease progression; regulating diverse functions viz. metabolism, signaling, redox homeostasis, transport, stress-response, protein folding, modification and degradation, development. Metabolome study indicated differential regulation of organic acid, amino acids, and carbohydrates paralleling with the proteomics analysis. Further, we interrogated the proteome data using network analysis that identified two significant functional protein hubs centered around malate dehydrogenase, T-complex protein 1 subunit gamma, and ATP synthase beta. This study reports, for the first-time, kinetically controlled patho-stress responsive protein network during post-harvest storage in a sink tissue, particularly fruit and constitute the basis toward understanding the onset and context of disease signaling and metabolic pathway alterations. The network representation may facilitate the prioritization of candidate proteins for quality improvement in storage organ.
This work was supported by grants from National Institute of Plant Genome Research, New Delhi, India and Department of Biotechnology, Government of India (No. BT/HRD/35/01/05/2013) to SC. KN is the recipient of post-doctoral fellowship from Department of Biotechnology (DBT), Government of India. RG and AS are the recipients of pre-doctoral fellowship from the Council of Scientific and Industrial research (CSIR), Government of India. PJ is the recipient of pre-doctoral fellowship from the University Grants Commission (UGC), Government of India. Authors also thank Mr. Jasbeer Singh for illustrations and graphical representations in the manuscript.
 
Date 2016-08-11T09:39:41Z
2016-08-11T09:39:41Z
2016
 
Type Article
 
Identifier Front. Plant Sc., 7: 1034
1664-462X
http://172.16.0.77:8080/jspui/handle/123456789/673
http://journal.frontiersin.org/article/10.3389/fpls.2016.01034/full
10.3389/fpls.2016.01034
 
Language en_US
 
Publisher Frontiers Media S.A.