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Comparative proteomics of oxalate downregulated tomatoes points toward cross talk of signal components and metabolic consequences during post-harvest storage

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Title Comparative proteomics of oxalate downregulated tomatoes points toward cross talk of signal components and metabolic consequences during post-harvest storage
 
Creator Narula, Kanika
Ghosh, Sudip
Aggarwal, Pooja R.
Sinha, Arunima
Chakraborty, Niranjan
Chakraborty, Subhra
 
Subject post-harvest storage
2-DE coupled mass spectrometry
comparative proteomics
protein network
tomato fruit
shelf-life
 
Description Accepted date: 18 July 2016
Fruits of angiosperms evolved intricate regulatory machinery for sensorial attributes and storage quality after harvesting. Organic acid composition of storage organs forms the molecular and biochemical basis of organoleptic and nutritional qualities with metabolic specialization. Of these, oxalic acid (OA), determines the post-harvest quality in fruits. Tomato (Solanum lycopersicum) fruit has distinctive feature to undergo a shift from heterotrophic metabolism to carbon assimilation partitioning during storage. We have earlier shown that decarboxylative degradation of OA by FvOXDC leads to acid homeostasis besides increased fungal tolerance in E8.2-OXDC tomato. Here, we elucidate the metabolic consequences of oxalate down-regulation and molecular mechanisms that determine organoleptic features, signaling and hormonal regulation in E8.2-OXDC fruit during post-harvest storage. A comparative proteomics approach has been applied between wild-type and E8.2-OXDC tomato in temporal manner. The MS/MS analyses led to the identification of 32 and 39 differentially abundant proteins associated with primary and secondary metabolism, assimilation, biogenesis, and development in wild-type and E8.2-OXDC tomatoes, respectively. Next, we interrogated the proteome data using correlation network analysis that identified significant functional hubs pointing toward storage related coinciding processes through a common mechanism of function and modulation. Furthermore, physiochemical analyses exhibited reduced oxalic acid content with concomitant increase in citric acid, lycopene and marginal decrease in malic acid in E8.2-OXDC fruit. Nevertheless, E8.2-OXDC fruit maintained an optimal pH and a steady state acid pool. These might contribute to reorganization of pectin constituent, reduced membrane leakage and improved fruit firmness in E8.2-OXDC fruit with that of wild-type tomato during storage. Collectively, our study provides insights into kinetically controlled protein network, identified regulatory module for pathway formulation and provide basis toward understanding the context of storage quality maintenance as a consequence of oxalate downregulation in the sink organ.
This work was supported by grants from National Institute of Plant Genome Research, New Delhi, India to SC, KN is the recipient of post-doctoral fellowship from Department of Biotechnology (DBT), Govt. of India. PA and AS are the recipients of pre-doctoral fellowship from the Council of Scientific and Industrial research (CSIR), Govt. of India.
 
Date 2016-08-26T07:19:14Z
2016-08-26T07:19:14Z
2016
 
Type Article
 
Identifier Front. Plant Sc., 7: 1147
1664-462X
http://172.16.0.77:8080/jspui/handle/123456789/676
http://journal.frontiersin.org/article/10.3389/fpls.2016.01147/full
10.3389/fpls.2016.01147
 
Language en_US
 
Publisher Frontiers Media S.A.