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Genome-wide analysis of heat shock proteins in C4 model, foxtail millet identifies potential candidates for crop improvement under abiotic stress

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Title Genome-wide analysis of heat shock proteins in C4 model, foxtail millet identifies potential candidates for crop improvement under abiotic stress
 
Creator Singh, Roshan Kumar
Jaishankar, Jananee
Muthamilarasan, Mehanathan
Shweta, Shweta
Dangi, Anand
Prasad, Manoj
 
Subject Abiotic stress
Heat
foxtail millet
crop improvement
 
Description Accepted date: 10 August 2016
Heat shock proteins (HSPs) perform significant roles in conferring abiotic stress tolerance to crop plants. In view of this, HSPs and their encoding genes were extensively characterized in several plant species; however, understanding their structure, organization, evolution and expression profiling in a naturally stress tolerant crop is necessary to delineate their precise roles in stress-responsive molecular machinery. In this context, the present study has been performed in C4 panicoid model, foxtail millet, which resulted in identification of 20, 9, 27, 20 and 37 genes belonging to SiHSP100, SiHSP90, SiHSP70, SiHSP60 and SisHSP families, respectively. Comprehensive in silico characterization of these genes followed by their expression profiling in response to dehydration, heat, salinity and cold stresses in foxtail millet cultivars contrastingly differing in stress tolerance revealed significant upregulation of several genes in tolerant cultivar. SisHSP-27 showed substantial higher expression in response to heat stress in tolerant cultivar, and its over-expression in yeast system conferred tolerance to several abiotic stresses. Methylation analysis of SiHSP genes suggested that, in susceptible cultivar, higher levels of methylation might be the reason for reduced expression of these genes during stress. Altogether, the study provides novel clues on the role of HSPs in conferring stress tolerance.
Authors’ work in foxtail millet genomics is funded by the Core Grant of National Institute of Plant Genome Research (NIPGR), New Delhi, India, which is gratefully acknowledged. R.S. and M.M. acknowledge Council of Scientific and Industrial Research and University Grants Commission, New Delhi, India, respectively, for providing Research Fellowships. Authors thank Dr. Chandra Bhan Yadav, NIPGR for his assistance in analyzing methylation data. The assistance received from Mr. Rohit Khandelwal in critical reading of this manuscript is appreciated.
 
Date 2016-09-06T11:44:59Z
2016-09-06T11:44:59Z
2016
 
Type Article
 
Identifier Scientific Reports, 6: 32641
2045-2322
http://172.16.0.77:8080/jspui/handle/123456789/678
http://www.nature.com/articles/srep32641
10.1038/srep32641
 
Language en_US
 
Publisher Nature Publishing Group