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Comprehensive analysis of SET domain gene family in foxtail millet identifies the putative role of SiSET14 in abiotic stress tolerance

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Title Comprehensive analysis of SET domain gene family in foxtail millet identifies the putative role of SiSET14 in abiotic stress tolerance
 
Creator Yadav, Chandra Bhan
Muthamilarasan, Mehanathan
Dangi, Anand
Shweta, Shweta
Prasad, Manoj
 
Subject Abiotic stress
Drought
foxtail millet
SiSET14
 
Description Accepted date: 10 August 2016
SET domain-containing genes catalyse histone lysine methylation, which alters chromatin structure and regulates the transcription of genes that are involved in various developmental and physiological processes. The present study identified 53 SET domain-containing genes in C4 panicoid model, foxtail millet (Setaria italica) and the genes were physically mapped onto nine chromosomes. Phylogenetic and structural analyses classified SiSET proteins into five classes (I–V). RNA-seq derived expression profiling showed that SiSET genes were differentially expressed in four tissues namely, leaf, root, stem and spica. Expression analyses using qRT-PCR was performed for 21 SiSET genes under different abiotic stress and hormonal treatments, which showed differential expression of these genes during late phase of stress and hormonal treatments. Significant upregulation of SiSET gene was observed during cold stress, which has been confirmed by over-expressing a candidate gene, SiSET14 in yeast. Interestingly, hypermethylation was observed in gene body of highly differentially expressed genes, whereas methylation event was completely absent in their transcription start sites. This suggested the occurrence of demethylation events during various abiotic stresses, which enhance the gene expression. Altogether, the present study would serve as a base for further functional characterization of SiSET genes towards understanding their molecular roles in conferring stress tolerance.
Authors’ work in foxtail millet genomics is funded by the Core Grant of National Institute of Plant Genome Research, New Delhi, India, which is gratefully acknowledged. C.B.Y. acknowledges the Science and Engineering Research Board, Department of Science and Technology, Govt. of India, India for providing him the Young Scientist Research Grant (File No. YSS/2015/000287). M.M. acknowledges the University Grants Commission, New Delhi, India for providing Research Fellowship.
 
Date 2016-09-06T12:00:21Z
2016-09-06T12:00:21Z
2016
 
Type Article
 
Identifier Scientific Reports, 6: 32621
2045-2322
http://172.16.0.77:8080/jspui/handle/123456789/679
http://www.nature.com/articles/srep32621
10.1038/srep32621
 
Language en_US
 
Publisher Nature Publishing Group