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Differentially expressed galactinol synthase(s) in chickpea are implicated in seed vigor and longevity by limiting the age induced ROS accumulation

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Title Differentially expressed galactinol synthase(s) in chickpea are implicated in seed vigor and longevity by limiting the age induced ROS accumulation
 
Creator Salvi, Prafull
Saxena, Saurabh Chandra
Petla, Bhanu Prakash
Kamble, Nitin Uttam
Kaur, Harmeet
Verma, Pooja
Rao, Venkateswara
Ghosh, Shraboni
Majee, Manoj
 
Subject Abiotic
Plant molecular biology
chickpea
galactinol synthase(s)
 
Description Accepted date: 23 September 2016
Galactinol synthase (GolS) catalyzes the first and rate limiting step of Raffinose Family Oligosaccharide (RFO) biosynthetic pathway, which is a highly specialized metabolic event in plants. Increased accumulation of galactinol and RFOs in seeds have been reported in few plant species, however their precise role in seed vigor and longevity remain elusive. In present study, we have shown that galactinol synthase activity as well as galactinol and raffinose content progressively increase as seed development proceeds and become highly abundant in pod and mature dry seeds, which gradually decline as seed germination progresses in chickpea (Cicer arietinum). Furthermore, artificial aging also stimulates galactinol synthase activity and consequent galactinol and raffinose accumulation in seed. Molecular analysis revealed that GolS in chickpea are encoded by two divergent genes (CaGolS1 and CaGolS2) which potentially encode five CaGolS isoforms through alternative splicing. Biochemical analysis showed that only two isoforms (CaGolS1 and CaGolS2) are biochemically active with similar yet distinct biochemical properties. CaGolS1 and CaGolS2 are differentially regulated in different organs, during seed development and germination however exhibit similar subcellular localization. Furthermore, seed-specific overexpression of CaGolS1 and CaGolS2 in Arabidopsis results improved seed vigor and longevity through limiting the age induced excess ROS and consequent lipid peroxidation.
This work was supported by the grant (BT/AGR/CG-PhaseII/01/2014) from Department of Biotechnology, Government of India and NIPGR core grant. P.S., B.P., N.K.,V.R., SG thank Council of Scientific and Industrial Research and University Grant Commission, Government of India, for research fellowships. We thank technicians at NIPGR central instrumentation facility.
 
Date 2016-10-17T06:57:24Z
2016-10-17T06:57:24Z
2016
 
Type Article
 
Identifier Scientific Reports, 6: 35088
2045-2322
http://59.163.192.83:8080/jspui/handle/123456789/688
http://www.nature.com/articles/srep35088
10.1038/srep35088
 
Language en_US
 
Publisher Nature Publishing Group