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PiHOG1, a stress regulator MAP kinase from the root endophyte fungus Piriformospora indica, confers salinity stress tolerance in rice plants

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Title PiHOG1, a stress regulator MAP kinase from the root endophyte fungus Piriformospora indica, confers salinity stress tolerance in rice plants
 
Creator Jogawat, Abhimanyu
Vadassery, Jyothilakshmi
Verma, Nidhi
Oelmüller, Ralf
Dua, Meenakshi
Nevo, Eviatar
Johri, Atul Kumar
 
Subject Microbiology
Molecular biology
Piriformospora indica
MAP kinase
 
Description Accepted date: 20 October 2016
In this study, yeast HOG1 homologue from the root endophyte Piriformospora indica (PiHOG1) was isolated and functionally characterized. Functional expression of PiHOG1 in S. cerevisiae ∆hog1 mutant restored osmotolerance under high osmotic stress. Knockdown (KD) transformants of PiHOG1 generated by RNA interference in P. indica showed that genes for the HOG pathway, osmoresponse and salinity tolerance were less stimulated in KD-PiHOG1 compared to the wild-type under salinity stress. Furthermore, KD lines are impaired in the colonization of rice roots under salinity stress of 200 mM NaCl, and the biomass of the host plants, their shoot and root lengths, root number, photosynthetic pigment and proline contents were reduced as compared to rice plants colonized by WT P. indica. Therefore, PiHOG1 is critical for root colonisation, salinity tolerance and the performance of the host plant under salinity stress. Moreover, downregulation of PiHOG1 resulted not only in reduced and delayed phosphorylation of the remaining PiHOG1 protein in colonized salinity-stressed rice roots, but also in the downregulation of the upstream MAP kinase genes PiPBS2 and PiSSK2 involved in salinity tolerance signalling in the fungus. Our data demonstrate that PiHOG1 is not only involved in the salinity response of P. indica, but also helping host plant to overcome salinity stress.
AKJ, MD and JV acknowledge the financial support from Department of Science and Technology, Govt. of India, the Council of Scientific and Industrial Research, Department of Biotechnology and JNU for providing the Capacity build up and UPOE-II fund. RO was supported by a CRC1127 (Germany) grant. AJ thanks Council of Scientific and Industrial Research, Govt. of India for research fellowships. We thank the Central Instrumentation facility of School of Life Sciences, JNU, Dr. Alga Zuccaro, Max Plank Institute for Terrestial Microbiology, Marburg, Germany, for the pRNAi vector.
 
Date 2016-11-18T09:39:37Z
2016-11-18T09:39:37Z
2016
 
Type Article
 
Identifier Scientific Reports, 6: 36765
2045-2322
http://59.163.192.83:8080/jspui/handle/123456789/692
http://www.nature.com/articles/srep36765
10.1038/srep36765
 
Language en_US
 
Publisher Nature Publishing Group