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UV‐B activates a ‘group A’ mitogen activated protein kinase in Oryza sativa

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Title UV‐B activates a ‘group A’ mitogen activated protein kinase in Oryza sativa
 
Creator Wankhede, Dhammaprakash Pandhari
Singh, Pallavi
Jaggi, Monika
Rao, Kudupudi Prabhakara
Raina, Susheel Kumar
Sinha, Alok Krishna
 
Subject UV-B stress
MAP kinase
OsWRKY89
Rice
 
Description Accepted date: 31 January 2016
Increased level of Ultra violet-B radiation at earth’s surface has several deleterious consequences for plants and ecosystems. Higher UV-B level affects crop plants in several ways and also gives penalty in terms of crop yield. With changing climatic conditions it is crucial to elucidate signal transduction pathway involved in UV-B stress. Here, involvement of mitogen activated protein kinases (MAPKs) in UV-B stress was investigated in rice (Oryza sativa). Transcripts profiling of all 15 rice MAPKs have shown UV-B induced expressions of a few MAPK genes including OsMPK3, OsMPK6, OsMPK4, OsMPK17-1, OsMPK17-2, OsMPK20-1, OsMPK20-4 and OsMPK20-5. In-gel kinase assay as well as immuno-kinase assay showed activation of single MAPK of size ~45 kDa in response to UV-B treatment. Further UV-B responsive activity of rice MAPKs, OsMPK3, OsMPK4 and OsMPK6 was checked using antibodies for the respective orthologs in Arabidopsis which indicated activation of OsMPK3 in UV-B stress. Additionally, GST: OsMPK3 protein showed UV responsive phosphorylation when incubated with crude protein extract from UV exposed plants in an in-vitro phosphorylation assay. These results indicate activation of OsMPK3 in UV-B stress. Further, possible involvement of UV-B responsive OsWRKY89 downstream of MAPK cascade was studied. It was observed that staurosporin and other specific chemical inhibitors of MAPK could attenuate UV-B induced expression of OsWRKY89. OsMPK3 was also found to interact with OsWRKY89 in yeast two-hybrid assay, although the interaction was weak in nature. The present work gives an account of MAPKs in UV-B stress in rice.
DPW, KPR thank University Grant Commission, India and MJ, SKR, PS thank Council of Scientific and Industrial Research, India for fellowships. This work is supported by core grant of National Institute of Plant Genome Research, New Delhi, India from Department of Biotechnology, India.
 
Date 2016-11-25T06:32:11Z
2016-11-25T06:32:11Z
2016
 
Type Article
 
Identifier J. Plant Biochem. Biotechnol., 25(4): 392–399
0974-1275
http://59.163.192.83:8080/jspui/handle/123456789/694
http://link.springer.com/article/10.1007/s13562-016-0351-0
10.1007/s13562-016-0351-0
 
Language en_US
 
Publisher Springer