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Transcriptomic analysis suggests a key role for SQUAMOSA PROMOTER BINDING PROTEIN LIKE, NAC and YUCCA genes in the heteroblastic development of the temperate rainforest tree Gevuina avellana (Proteaceae)

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Title Transcriptomic analysis suggests a key role for SQUAMOSA PROMOTER BINDING PROTEIN LIKE, NAC and YUCCA genes in the heteroblastic development of the temperate rainforest tree Gevuina avellana (Proteaceae)
 
Creator Ostria-Gallardo, Enrique
Ranjan, Aashish
Zumstein, Kristina
Chitwood, Daniel H.
Kumar, Ravi
Townsley, Brad T.
Ichihashi, Yasunori
Corcuera, Luis J.
Sinha, Neelima R.
 
Subject Gevuina avellana
RNA-seq
heteroblasty
light availability
temperate rainforest
transcriptional regulation
 
Description Accepted date: 19 October 2015
Heteroblasty, the temporal development of the meristem, can produce diverse leaf shapes within a plant. Gevuina avellana, a tree from the South American temperate rainforest shows strong heteroblasty affecting leaf shape, transitioning from juvenile simple leaves to highly pinnate adult leaves. Light availability within the forest canopy also modulates its leaf size and complexity. Here we studied how the interaction between the light environment and the heteroblastic progression of leaves is coordinated in this species. We used RNA-seq on the Illumina platform to compare the range of transcriptional responses in leaf primordia of G. avellana at different heteroblastic stages and growing under different light environments. We found a steady up-regulation of SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL), NAC, YUCCA and AGAMOUS-LIKE genes associated with increases in age, leaf complexity, and light availability. In contrast, expression of TCP, TPR and KNOTTED1 homeobox genes showed a sustained down-regulation. Additionally, genes involved in auxin synthesis/transport and jasmonate activity were differentially expressed, indicating an active regulation of processes controlled by these hormones. Our large-scale transcriptional analysis of the leaf primordia of G. avellana sheds light on the integration of internal and external cues during heteroblastic development in this species.
E.O-G. thanks the Chilean National Commission for Scientific and Technological Research for a doctoral fellowship, and the internship grant supported by Universidad de Concepción, project Mecesup UCO0708. Also thanks are due to Dr León Bravo, Holly Forbes, and Alejandro Navarrete for fieldwork, and Katalapi Park for excellent research field facilities. We thank the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley, supported by NIH S10 Instrumentation Grants S10RR029668 and S10RR027303. Part of the work was supported by NSF PGRP grant IOS-1238243 (to Julia Bailey-Serres, N.R.S., Siobhan Brady and Roger Deal). This work used computational resources or cyber-infrastructure provided by the iPlant Collaborative (http://www.iplantcollaborative.org). The iPlant Collaborative is funded by National Science Foundation Grant DBI-0735191.
 
Date 2016-12-20T07:11:46Z
2016-12-20T07:11:46Z
2016
 
Type Article
 
Identifier New Phytol., 210(2): 694-708
1469-8137
http://59.163.192.83:8080/jspui/handle/123456789/701
http://onlinelibrary.wiley.com/doi/10.1111/nph.13776/abstract;jsessionid=2724ACD6525580103B2192079C1A8529.f04t03
10.1111/nph.13776
 
Language en_US
 
Publisher John Wiley & Sons