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A novel FIKK kinase regulates the development of mosquito and liver stages of the malaria

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Title A novel FIKK kinase regulates the development of mosquito and liver stages of the malaria
 
Creator Jaijyan, Dabbu Kumar
Verma, Praveen K.
Singh, Agam Prasad
 
Subject Parasite development
Transcriptomics
malaria
FIKK kinase
 
Description Accepted date: 22 November 2016
Protein phosphorylation is the most important post-translational event in the regulation of various essential signaling pathways in a cell. Here, we show the functional characterization of a FIKK family protein kinase of the rodent malaria parasite (PbMLFK), which is expressed only in mosquito and liver stages and contains two functional C-terminal PEXEL motifs. We demonstrate that this protein plays a role in mosquito and liver stages of parasite growth. The oocysts of PbMLFK-deficient parasites produced 4-fold fewer sporozoites. In the liver of infected mice, PbMLFK-deficient parasites grew 100-fold less than did wild type parasites. We also show that the C-terminal domain of this protein has a functional serine-threonine kinase and that its activity was inhibited by a known PKA inhibitor. Transcriptome analysis of infected host cells suggests that in absence of this protein expression of the 288 host mRNAs are perturbed which are primarily associated with the immune system, cell cycle and metabolism.
We thank Pradeep Kumar Tiwari and Vivek Kumar Pandey for their technical assistance in mosquito breeding and animal handling. We thank Dr. Arnab Mukhopadhyay and Dr. Awadhesh Pandit for their help in collecting the NGS data at the NII core facility. We also thank Vikash Kumar at the NII central FACS facility for cell sorting.
 
Date 2016-12-26T10:35:59Z
2016-12-26T10:35:59Z
2016
 
Type Article
 
Identifier Scientific Reports, 6: 39285
2045-2322
http://59.163.192.83:8080/jspui/handle/123456789/703
http://www.nature.com/articles/srep39285?WT.feed_name=subjects_molecular-biology
10.1038/srep39285
 
Language en_US
 
Publisher Nature Publishing Group