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Root transcriptome of two contrasting indica rice cultivars uncovers regulators of root development and physiological responses

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Title Root transcriptome of two contrasting indica rice cultivars uncovers regulators of root development and physiological responses
 
Creator Singh, Alka
Kumar, Pramod
Gautam, Vibhav
Rengasamy, Balakrishnan
Adhikari, Bijan
Udayakumar, Makarla
Sarkar, Ananda K.
 
Subject Plant molecular biology
Plant physiology
indica rice
root development
Root transcriptome
 
Description Accepted date: 21 November 2016
The huge variation in root system architecture (RSA) among different rice (Oryza sativa) cultivars is conferred by their genetic makeup and different growth or climatic conditions. Unlike model plant Arabidopsis, the molecular basis of such variation in RSA is very poorly understood in rice. Cultivars with stable variation are valuable resources for identification of genes involved in RSA and related physiological traits. We have screened for RSA and identified two such indica rice cultivars, IR-64 (OsAS83) and IET-16348 (OsAS84), with stable contrasting RSA. OsAS84 produces robust RSA with more crown roots, lateral roots and root hairs than OsAS83. Using comparative root transcriptome analysis of these cultivars, we identified genes related to root development and different physiological responses like abiotic stress responses, hormone signaling, and nutrient acquisition or transport. The two cultivars differ in their response to salinity/dehydration stresses, phosphate/nitrogen deficiency, and different phytohormones. Differential expression of genes involved in salinity or dehydration response, nitrogen (N) transport, phosphate (Pi) starvation signaling, hormone signaling and root development underlies more resistance of OsAS84 towards abiotic stresses, Pi or N deficiency and its robust RSA. Thus our study uncovers gene-network involved in root development and abiotic stress responses in rice.
We thank Department of Biotechnology (DBT), India (grant no. BT/PR3292/AGR/2/811/2011) and National Institute of Plant Genome Research (NIPGR) for financial support and central instrument facility of NIPGR. Fellowship to AS was provided by DBT and fellowship to PK and VG was provided by Council of Scientific and Industrial Research (CSIR), India. BR was supported by above DBT grant and NIPGR fellowship. We also thank Suvakanta Barik and M. Karthikeyan for help in preliminary stage of the work.
 
Date 2016-12-27T11:10:45Z
2016-12-27T11:10:45Z
2016
 
Type Article
 
Identifier Scientific Reports, 6: 39266
2045-2322
http://59.163.192.83:8080/jspui/handle/123456789/710
http://www.nature.com/articles/srep39266
10.1038/srep39266
 
Language en_US
 
Publisher Nature Publishing Group