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Mass production of Ajmalicine by bioreactor cultivation of hairy roots of Catharanthus roseus

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Title Mass production of Ajmalicine by bioreactor cultivation of hairy roots of Catharanthus roseus
 
Creator Thakore, Dhara
Srivastava, Ashok Kumar
Sinha, Alok Krishna
 
Subject Hairy root
Ajmalicine
Bubble column bioreactor
Modified bubble column
Rotating drum bioreactor
Catharanthus roseus
 
Description Accepted date: 14 December 2016
Catharanthus roseus has been known to produce Ajmalicine which is used for treatment of circulatory disorders. Significantly low content of the drug in the natural plant necessitates development of alternative production protocols. Hairy root propagation was considered as a viable alternative. Mass cultivation of the hairy root culture was attempted in several bioreactor configurations; Bubble column, Rotating drum bioreactor, Modified Bubble column with polypropylene (PP) mesh support and Modified Bubble column with Polyurethane foam (PUF) support. Important factors like medium composition, inoculum density, illumination period and aeration rates were optimized before mass propagation of hairy roots. From the data gleaned, the cultivation in rotating drum bioreactor resulted in ajmalicine content of only 4.6 ± 0.4 mg/l. However, the ajmalicine concentration surpassed even that obtained in shake flask (with 34 ± 2.3 mg/l) in a customized bioreactor wherein the hairy roots were anchored onto a polyurethane foam, the highest reported in this scale of cultivation.
 
Date 2017-01-12T10:23:53Z
2017-01-12T10:23:53Z
2017
 
Type Article
 
Identifier Biochemical Engineering J., 119: 84-91
1369-703X
http://59.163.192.83:8080/jspui/handle/123456789/715
http://www.sciencedirect.com/science/article/pii/S1369703X16303461
http://dx.doi.org/10.1016/j.bej.2016.12.010
 
Language en_US
 
Publisher Elsevier B.V.