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Improvement of phosphate acquisition and utilization by a secretory purple acid phosphatase (OsPAP21b) in rice

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Title Improvement of phosphate acquisition and utilization by a secretory purple acid phosphatase (OsPAP21b) in rice
 
Creator Mehra, Poonam
Pandey, Bipin Kumar
Giri, Jitender
 
Subject Acid phosphatase
Organic-phosphates
P-use-efficiency
Overexpression
RNAi
Root
Secretory protein
 
Description Accepted date: 18 January 2017
Phosphate (Pi) deficiency in soil system is a limiting factor for rice growth and yield. Majority of the soil Phosphorus (P) is organic in nature; not readily available for root uptake. Low Pi inducible purple acid phosphatases (PAPs) are hypothesized to enhance the availability of Pi in soil and cellular system. However, information on molecular and physiological roles of rice PAPs is very limited. Here, we demonstrate the role of a novel rice PAP, OsPAP21b in improving plant utilization of organic-P. OsPAP21b was found to be under the transcriptional control of OsPHR2 and strictly regulated by plant Pi-status at both transcript and protein levels. Biochemically, OsPAP21b showed hydrolysis of several organophosphates at acidic pH and possessed sufficient thermostability befitting for high temperature rice ecosystems with acidic soils. Interestingly, OsPAP21b was revealed to be a secretory PAP and encodes a distinguishable major APase (acid phosphatase) isoform under low Pi in roots. Further, OsPAP21b overexpressing transgenics showed increased biomass, APase activity and P content both in hydroponics supplemented with organic-P sources and soil containing organic manure as sole P source. Additionally, overexpression lines depicted increased root length, biomass and lateral roots under low Pi while RNAi lines showed reduced root length and biomass as compared to WT. In light of these evidences, present study strongly proposes OsPAP21b as a useful candidate for improving Pi acquisition and utilization in rice.
This work was supported by research grant, BT/PR3299/AGR/2/813/2011 of DBT, Government of India and NIPGR core grant. P.M. and B.K.P acknowledge research fellowships by CSIR and DBT, respectively. The authors declare no conflict of interest.
 
Date 2017-01-27T07:03:42Z
2017-01-27T07:03:42Z
2017
 
Type Article
 
Identifier Plant Biotechnology Journal, 15(8): 1054-1067
1467-7652
http://59.163.192.83:8080/jspui/handle/123456789/719
http://onlinelibrary.wiley.com/doi/10.1111/pbi.12699/abstract;jsessionid=73AE86DA2C900C355469B65A744DD673.f02t03?systemMessage=WOL+Usage+report+download+page+will+be+unavailable+on+Friday+27th+January+2017+at+23%3A00+GMT%2F+18%3A00+EST%2F+07%3A00+SGT+%28Saturday+28th+Jan+for+SGT%29++for+up+to+2+hours+due+to+essential+server+maintenance.+Apologies+for+the+inconvenience.
10.1111/pbi.12699
 
Language en_US
 
Publisher John Wiley & Sons