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Ralstonia solanacearum virulence in tomato seedlings inoculated by leaf clipping

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Title Ralstonia solanacearum virulence in tomato seedlings inoculated by leaf clipping
 
Creator Kumar, R.
Barman, A.
Phukan, T.
Kabyashree, K.
Singh, N.
Jha, Gopaljee
Sonti, R.V.
Genin, S.
Ray, R.K.
 
Subject bacterial wilt
leaf-clip inoculation
Ralstonia solanacearum
tomato seedling infection
 
Description Accepted date: 30 September 2016
Ralstonia solanacearum is a phytopathogenic bacterium that colonizes the xylem vessels of host plants leading to a lethal wilt disease. Although several studies have investigated the virulence of R. solanacearum on adult host plants, infection studies of this pathogen on the seedling stages of hosts are less common. In a preliminary observation, inoculation of R. solanacearum F1C1 on 6- to 7-day-old tomato seedlings by a simple leaf-clip strategy resulted in a lethal pathogenic condition in seedlings that eventually killed these seedlings within a week post-inoculation. This prompted testing of the effect of this inoculation technique in seedlings from different cultivars of tomato and similar results were obtained. Colonization and spread of the bacteria throughout the infected seedlings was demonstrated using gus-tagged R. solanacearum F1C1. The same method of inoculating tomato seedlings was used with R. solanacearum GMI1000 and independent mutants of R. solanacearum GMI1000, deficient in the virulence genes hrpB, hrpG, phcA and gspD. Wildtype R. solanacearum GMI1000 was found to be virulent on tomato seedlings, whereas the mutants were found to be non-virulent. This leaf-clip technique, for inoculation of tomato seedlings, has the potential to be a valuable approach, saving time, space, labour and costs.
The authors are very thankful to the anonymousreviewer for critical suggestions, leading to a better con-clusion of this work. In addition, the authors thankPatrick Barberis for helping to conduct experiments withtomato seedlings at LIPM, France, and Dr Nemo Peeters,LIPM, France for comments on the statistical analysis ofthe survivability study. This work was funded by aresearch grant under the twinning project (BT/301/NE/TBP/2012) from Department of Biotechnology, Govern-ment of India (to S.K.R., K.K. and R.V.S.); an Indo-French project grant (4800-B1) from Indo-French Centrefor the Promotion of Advanced Research, India (CEFI-PRA, to S.K.R., R.K. and S.G.); research fellowshipsfrom University Grants Commission, Government ofIndia (to A.B. and T.P.) and Department of Biotechnol-ogy, Government of India (to N.S.). S.K.R. is also sup-ported by funds from departmental projects UGC-SAP(DSR II), DST-FIST (Phase I) and DBT-StrengtheningNE. A part of this work has been presented as a posterin the 6th International Bacterial Wilt Symposium heldin Toulouse in July 2016. The authors declare there is noconflict of interest.
 
Date 2017-02-03T11:58:20Z
2017-02-03T11:58:20Z
2017
 
Type Article
 
Identifier Plant Pathol., 66(5): 835-841
1365-3059
http://59.163.192.83:8080/jspui/handle/123456789/721
http://onlinelibrary.wiley.com/doi/10.1111/ppa.12628/abstract
10.1111/ppa.12628
 
Language en_US
 
Publisher John Wiley & Sons