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Sirtinol, a Sir2 protein inhibitor, affects stem cell maintenance and root development in Arabidopsis thaliana by modulating auxin-cytokinin signaling components

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Title Sirtinol, a Sir2 protein inhibitor, affects stem cell maintenance and root development in Arabidopsis thaliana by modulating auxin-cytokinin signaling components
 
Creator Singh, Sharmila
Singh, Alka
Yadav, Sandeep
Gautam, Vibhav
Singh, Archita
Sarkar, Ananda K.
 
Subject Plant molecular biology
Plant biotechnology
Arabidopsis thaliana
Sirtinol
Sir2
auxin-cytokinin
root development
 
Description Accepted date: 09 January 2017
In Arabidopsis thaliana, besides several key transcription factors and chromatin modifiers, phytohormones auxin and cytokinin play pivotal role in shoot and root meristem maintenance, and lateral root (LR) development. Sirtinol, a chemical inhibitor of Sir2 proteins, is known to promote some auxin induced phenotypes in Arabidopsis. However, its effect on plant stem cell maintenance or organ formation remained unaddressed. Here we show that sirtinol affects meristem maintenance by altering the expression of key stem cell regulators, cell division and differentiation by modulating both auxin and cytokinin signaling in Arabidopsis thaliana. The expression of shoot stem cell niche related genes WUSCHEL (WUS) and CLAVATA3 (CLV3) was upregulated, whereas SHOOT MERISTEMLESS (STM) was downregulated in sirtinol treated seedlings. The expression level and domain of key root stem cell regulators PLETHORA (PLTs) and WUS-Related Homeobox 5 (WOX5) were altered in sirtinol treated roots. Sirtinol affects LR development by disturbing proper auxin transport and maxima formation, similar to 2,4-dichlorophenoxyacetic acid (2,4-D). Sirtinol also affects LR formation by altering cytokinin biosynthesis and signaling genes in roots. Therefore, sirtinol affects shoot and root growth, meristem maintenance and LR development by altering the expression of cytokinin-auxin signaling components, and regulators of stem cells, meristems, and LRs.
We acknowledge internal funding and Central Instrument Facility of National Institute of Plant Genome Research (NIPGR) to carry out this work. We thank Dr. Peter Doerner (The University of Edinburgh; CyclinB1;1:CDB-GUS), Dr. Tom Beeckman (Department of Plant Systems Biology, VIB, Belgium; CyclinB1;1:GUS and GATA23:GUS), Dr. Kalika Prasad (IISER, Thiruvananthapuram; PLT1:PLT1-YFP and PLT2:PLT2-YFP) and Dr. Klaus Palme and Dr. Franck Ditengou (University of Freiburg; QC184) for kindly providing seeds. We also thank ABRC for other Arabidopsis seeds. We thank Prof. Ben Scheres (Wageningen University, Netherlands) for valuable suggestions. We thank Department of Biotechnology, Council of Industrial and Scientific Research and University Grants Commission for providing fellowship to AS (DBT), SS, VG, ArS (CSIR), and SY (UGC) respectively.
 
Date 2017-02-17T05:01:48Z
2017-02-17T05:01:48Z
2017
 
Type Article
 
Identifier Scientific Reports, 7: 42450
2045-2322
http://59.163.192.83:8080/jspui/handle/123456789/723
http://www.nature.com/articles/srep42450
10.1038/srep42450
 
Language en_US
 
Publisher Nature Publishing Group