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bHLH142 regulates various metabolic pathway-related genes to affect pollen development and anther dehiscence in rice

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Title bHLH142 regulates various metabolic pathway-related genes to affect pollen development and anther dehiscence in rice
 
Creator Ranjan, Rajeev
Khurana, Reema
Malik, Naveen
Badoni, Saurabh
Parida, Swarup K.
Kapoor, Sanjay
Tyagi, Akhilesh K.
 
Subject Plant molecular biology
Pollen
rice
anther dehiscence
metabolic pathway-related genes
 
Description Accepted date: 20 January 2017
Apposite development of anther and its dehiscence are important for the reproductive success of the flowering plants. Recently, bHLH142, a bHLH transcription factor encoding gene of rice has been found to show anther-specific expression and mutant analyses suggest its functions in regulating tapetum differentiation and degeneration during anther development. However, our study on protein level expression and gain-of-function phenotype revealed novel aspects of its regulation and function during anther development. Temporally dissimilar pattern of bHLH142 transcript and polypeptide accumulation suggested regulation of its expression beyond transcriptional level. Overexpression of bHLH142 in transgenic rice resulted in indehiscent anthers and aborted pollen grains. Defects in septum and stomium rupture caused anther indehiscence while pollen abortion phenotype attributed to abnormal degeneration of the tapetum. Furthermore, RNA-Seq-based transcriptome analysis of tetrad and mature pollen stage anthers of wild type and bHLH142OEplants suggested that it might regulate carbohydrate and lipid metabolism, cell wall modification, reactive oxygen species (ROS) homeostasis and cell death-related genes during rice anther development. Thus, bHLH142 is an anther-specific gene whose expression is regulated at transcriptional and post-transcriptional/translational levels. It plays a role in pollen maturation and anther dehiscence by regulating expression of various metabolic pathways-related genes.
This work is supported by grant from NIPGR, New Delhi and project grant from the DBT (Department of Biotechnology), Government of India. R.R. and N.M. acknowledge CSIR (Council of Scientific and Industrial Research) for Junior and Senior Research Fellowship awards. R.K. acknowledges UGC (University Grant Commission) for Dr. D.S. Kothari Post-Doctoral Fellowship award.
 
Date 2017-03-09T09:24:54Z
2017-03-09T09:24:54Z
2017
 
Type Article
 
Identifier Scientific Reports, 7: 43397
2045-2322
http://59.163.192.83:8080/jspui/handle/123456789/724
http://www.nature.com/articles/srep43397
10.1038/srep43397
 
Language en_US
 
Publisher Nature Publishing Group