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Interplay between auxin and cytokinin and its impact on mitogen activated protein kinase (MAPK)

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Title Interplay between auxin and cytokinin and its impact on mitogen activated protein kinase (MAPK)
 
Creator Singh, Pallavi
Sinha, Alok Krishna
 
Subject Auxin
Cytokinin
Mitogen activated protein kinase (MAPK)
MAPK assays
In-gel kinase assay
Immunoblotting
qRT-PCR
Root structure architecture (RSA)
 
Description Accepted date: 1 January 2017
Plant physiology, in particular, is governed by a repertoire of endogenous as well as environmental cues. Auxin and cytokinin constitute an indispensable phytohormonal system required for plant growth and development. Another pivotal aspect of plant physiological process that thoroughly affects various plant growth and developmental attributes is the signaling network, majorly comprising the canonical mitogen activated protein kinase (MAPK) cascade. Striking a fine balance between the phytohormonal and signaling components could be adopted as an intricate strategy by plants to counteract various stresses in question. Thus, a brief understanding of this multifaceted complex could be of use for delineating numerous plant physiological and developmental phenomena. Thus, the present section discusses the various MAPK related assays in context to auxin and cytokinin crosstalk. Briefly, this chapter outlines the discrete MAPK methods to better understand the fundamentals of MAPK signaling network in auxin and cytokinin treated rice seedlings. Further, various phenotypic, genomic as well as proteomic protocols are discussed for a better understanding of MAPK networks in the backdrop of auxin and cytokinin interplay.
 
Date 2017-03-09T09:36:53Z
2017-03-09T09:36:53Z
2017
 
Type Book chapter
 
Identifier Methods Mol. Biol., 1569: 93-100
978-1-4939-6831-2
http://59.163.192.83:8080/jspui/handle/123456789/725
https://link.springer.com/protocol/10.1007/978-1-4939-6831-2_7 ; http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-4939-6831-2_7
10.1007/978-1-4939-6831-2_7
 
Language en_US
 
Publisher Springer