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Concurrent drought stress and vascular pathogen infection induce common and distinct transcriptomic responses in chickpea

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Title Concurrent drought stress and vascular pathogen infection induce common and distinct transcriptomic responses in chickpea
 
Creator Sinha, Ranjita
Gupta, Aarti
Senthil-Kumar, Muthappa
 
Subject Ralstonia solanacearum
cellulose and lignin biosynthesis
combined stress
drought-pathogen stress
microarray
molecular response
unique response
 
Description Accepted date: 27 February 2017
Chickpea (Cicer arietinum); the second largest legume grown worldwide is prone to drought and various pathogen infections. These drought and pathogen stresses often occur concurrently in the field conditions. However, the molecular events in response to that are largely unknown. The present study examines the transcriptome dynamics in chickpea plants exposed to a combination of water-deficit stress and Ralstonia solanacearum infection. R. solanacearum is a potential wilt disease causing pathogen in chickpea. Drought stressed chickpea plants were infected with this pathogen and the plants were allowed to experience progressive drought with 2 and 4 days of R. solanacearum infection called short duration stress (SD stresses) and long duration stress (LD stresses), respectively. Our study showed that R. solanacearum multiplication decreased under SD-combined stress compared to SD-pathogen but there was no significant change in LD-combined stress compared to LD-pathogen. The microarray analysis during these conditions showed that 821 and 1039 differentially expressed genes (DEGs) were unique to SD- and LD-combined stresses, respectively, when compared with individual stress conditions. Three and fifteen genes were common among all the SD-stress treatments and LD-stress treatments, respectively. Genes involved in secondary cell wall biosynthesis, alkaloid biosynthesis, defense related proteins, and osmo-protectants were up-regulated during combined stress. The expression of genes involved in lignin and cellulose biosynthesis were specifically up-regulated in SD-combined, LD-combined, and LD-pathogen stress. A close transcriptomic association of LD-pathogen stress with SD-combined stress was observed in this study which indicates that R. solanacearum infection also exerts drought stress along with pathogen stress thus mimics combined stress effect. Furthermore the expression profiling of candidate genes using real-time quantitative PCR validated the microarray data. The study showed that down-regulation of defense-related genes during LD-combined stress resulted in an increased bacterial multiplication as compared to SD-combined stress. Overall, our study highlights a sub-set of DEGs uniquely expressed in response to combined stress, which serve as potential candidates for further functional characterization to delineate the molecular response of the plant to concurrent drought-pathogen stress.
We thank Science and Engineering Research Board (SERB), Department of Science and Technology (DST) for providing grant and fellowship for this study (SB/YS/LS-237/2013) to RS. Projects at MS-K lab are supported by National Institute of Plant Genome Research core funding and DBT-Ramalingaswami re-entry fellowship grant (BT/RLF/re-entry/23/2012).
 
Date 2017-04-10T08:57:23Z
2017-04-10T08:57:23Z
2017
 
Type Article
 
Identifier Front. Plant Sc., 8: 333
1664-462X
http://59.163.192.83:8080/jspui/handle/123456789/730
http://journal.frontiersin.org/article/10.3389/fpls.2017.00333/full
10.3389/fpls.2017.00333
 
Language en_US
 
Publisher Frontiers Media S.A.