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Extracellular matrix proteome and phosphoproteome of potato reveals functionally distinct and diverse canonical and non-canonical proteoforms

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Title Extracellular matrix proteome and phosphoproteome of potato reveals functionally distinct and diverse canonical and non-canonical proteoforms
 
Creator Elagamey, Eman
Narula, Kanika
Sinha, Arunima
Aggarwal, Pooja Rani
Ghosh, Sudip
Chakraborty, Niranjan
Chakraborty, Subhra
 
Subject ECM
proteoforms
phosphoproteome
potato
2-DE
protein interaction network
 
Description Accepted date: 13 June 2016
The extracellular matrix (ECM) has a molecular machinery composed of diverse proteins and proteoforms that combine properties of tensile strength with extensibility exhibiting growth-regulatory functions and self- and non-self-recognition. The identification of ECM proteoforms is the prerequisite towards a comprehensive understanding of biological functions accomplished by the outermost layer of the cell. Regulatory mechanisms of protein functions rely on post-translational modifications, phosphorylation in particular, affecting enzymatic activity, interaction, localization and stability. To investigate the ECM proteoforms, we have isolated the cell wall proteome and phosphoproteome of a tuberous crop, potato (Solanum tuberosum). LC-MS/MS analysis led to the identification of 38 proteins and 35 phosphoproteins of known and unknown functions. The findings may provide a better understanding of biochemical machinery and the integrated protein and phosphoprotein network of ECM for future functional studies of different developmental pathways and guidance cues in mechanosensing and integrity signaling.
This work was supported by grants from National Institute of Plant Genome Research,
New Delhi, India and Department of Biotechnology, Govt. of India (No. BT/HRD/35/01/05/2013) to S.C. E.E.
was the recipient of pre-doctoral fellowship from DBT-TWAS. K.N. is the recipient of post-doctoral fellowship from
Department of Biotechnology (DBT), Govt. of India. A.S. and P.A. are the recipients of pre-doctoral fellowship
from the Council of Scientific and Industrial research (CSIR), Govt. of India. Authors also thank Jasbeer Singh for
illustrations and graphical representations in the manuscript.
 
Date 2017-05-03T05:14:37Z
2017-05-03T05:14:37Z
2016
 
Type Article
 
Identifier Proteomes, 4(3): 20
2227-7382
http://59.163.192.83:8080/jspui/handle/123456789/738
http://www.mdpi.com/2227-7382/4/3/20
10.3390/proteomes4030020
 
Language en_US
 
Publisher MDPI AG