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Over-expression of OsHOX24 confers enhanced susceptibility to abiotic stresses in transgenic rice via modulating stress-responsive gene expression

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Title Over-expression of OsHOX24 confers enhanced susceptibility to abiotic stresses in transgenic rice via modulating stress-responsive gene expression
 
Creator Bhattacharjee, Annapurna
Sharma, Raghvendra
Jain, Mukesh
 
Subject abiotic stress
homeobox
microarray
over-expression
regulation
rice
transcription factor
transgenics
 
Description Accepted date: 07 April 2017
Homeobox transcription factors play critical roles in plant development and abiotic stress responses. In the present study, we raised rice transgenics over-expressing stress-responsive OsHOX24 gene (rice homeodomain-leucine zipper I sub-family member) and analyzed their response to various abiotic stresses at different stages of development. At the seed germination stage, rice transgenics over-expressing OsHOX24 exhibited enhanced sensitivity to abiotic stress conditions and abscisic acid as compared to wild-type (WT). OsHOX24 over-expression rice seedlings showed reduced root and shoot growth under salinity and desiccation stress (DS) conditions. Various physiological and phenotypic assays confirmed higher susceptibility of rice transgenics toward abiotic stresses as compared to WT at mature and reproductive stages of rice development too. Global gene expression profiling revealed differential regulation of several genes in the transgenic plants under control and DS conditions. Many of these differentially expressed genes were found to be involved in transcriptional regulatory activities, besides carbohydrate, nucleic acid and lipid metabolic processes and response to abiotic stress and hormones. Taken together, our findings highlighted the role of OsHOX24 in regulation of abiotic stress responses via modulating the expression of stress-responsive genes in rice.
AB acknowledges the award of research fellowship from the Council of Scientific and Industrial Research, New Delhi. We acknowledge the financial support of the Department of Science and Technology (F. No. SR/SO/PS/07/2011 from 2012 to 2015), Government of India, New Delhi and core grant from NIPGR.
 
Date 2017-05-22T09:48:57Z
2017-05-22T09:48:57Z
2017
 
Type Article
 
Identifier Frontiers in Plant Science, 8: 628
1664-462X
http://59.163.192.83:8080/jspui/handle/123456789/746
http://journal.frontiersin.org/article/10.3389/fpls.2017.00628/full
https://doi.org/10.3389/fpls.2017.00628
 
Language en_US
 
Publisher Frontiers Media S.A.