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CBL-interacting protein kinase 6 negatively regulates immune response to Pseudomonas syringae in Arabidopsis

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Title CBL-interacting protein kinase 6 negatively regulates immune response to Pseudomonas syringae in Arabidopsis
 
Creator Sardar, Atish
Nandi, Ashis Kumar
Chattopadhyay, Debasis
 
Subject Arabidopsis thaliana
CIPK6
ETI
PTI
Pseudomonas syringae
salicylic acid
 
Description Accepted date: 26 April 2017
Cytosolic calcium ion (Ca2+) is an essential mediator of the plant innate immune response. Here, we report that a calcium-regulated protein kinase Calcineurin B-like protein (CBL)-interacting protein kinase 6 (CIPK6) functions as a negative regulator of immunity against the bacterial pathogen Pseudomonas syringae in Arabidopsis thaliana. Arabidopsis lines with compromised expression of CIPK6 exhibited enhanced disease resistance to the bacterial pathogen and to P. syringae harboring certain but not all avirulent effectors, while restoration of CIPK6 expression resulted in abolition of resistance. Plants overexpressing CIPK6 were more susceptible to P. syringae. Enhanced resistance in the absence of CIPK6 was accompanied by increased accumulation of salicylic acid and elevated expression of defense marker genes. Salicylic acid accumulation was essential for improved immunity in the absence of CIPK6. CIPK6 negatively regulated the oxidative burst associated with perception of pathogen-associated microbial patterns (PAMPs) and bacterial effectors. Accelerated and enhanced activation of the mitogen-activated protein kinase cascade in response to bacterial and fungal elicitors was observed in the absence of CIPK6. The results of this study suggested that CIPK6 negatively regulates effector-triggered and PAMP-triggered immunity in Arabidopsis.
We acknowledge Dr J.W. Reed, Dr Vera Bonardi, and Dr Punita Nagpal of the Department of Biology, University of North Carolina, Chapel Hill (UNC) for their scientific and technical suggestions and materials. We also acknowledge Professor G.B. Martin, Cornel University, Ithaca, Dr V. Bonardi, Department of Biology, UNC, and Dr Saikat Bhattacharjee, Regional Centre for Biotechnology, Faridabad, India for plant lines and bacterial strains. Dr Alok K. Sinha of National Institute of Plant Genome Research, New Delhi is acknowledged for providing antibodies. AS acknowledges the Department of Biotechnology, Government of India for a fellowship.
 
Date 2017-05-29T09:15:14Z
2017-05-29T09:15:14Z
2017
 
Type Article
 
Identifier Journal of Experimental Botany, (In Press)
1460-2431
http://59.163.192.83:8080/jspui/handle/123456789/751
https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erx170
https://doi.org/10.1093/jxb/erx170
 
Language en_US
 
Publisher Oxford University Press