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Pattern of expression and interaction specificity of multiple G-protein beta (Gβ) subunit isoforms with their potential target proteins reveal functional dominance of BjuGβ1 in the allotetraploid Brassica juncea

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Title Pattern of expression and interaction specificity of multiple G-protein beta (Gβ) subunit isoforms with their potential target proteins reveal functional dominance of BjuGβ1 in the allotetraploid Brassica juncea
 
Creator Kumar, Roshan
Sharma, Aprajita
Chandel, Ishita
Bisht, Naveen C.
 
Subject Brassica
Heterotrimeric G-protein
Polyploid
Protein-protein interaction
Yeast two hybrid
 
Description Accepted date: 25 May 2017
Heterotrimeric G-protein, consisting Gα, Gβ and Gγ subunits, interacts with various upstream and downstream effector (target) proteins to regulate a large array of conserved and species-specific biological functions. The targets of G-protein components are recently reported in model plant Arabidopsis thaliana; however limited information is available from crop species. In this study, we utilized yeast two-hybrid (Y2H) assay to screen the diversity of interacting partners of multiple Gβ subunit isoforms from allotetraploid Brassica juncea, a globally important oilseed and vegetable crop. The three BjuGβ genes (BjuGβ1-3), resulted from whole genome triplication event in Brassica lineage, showed distinct expression profile during plant developmental stages with maximal transcript abundance during reproductive stages. Protein-protein interaction of three BjuGβ proteins (bait) against the Y2H cDNA library (prey) identified a total of 14 and 1 non-redundant targets for BjuGβ1 and BjuGβ2, whereas BjuGβ3 screening surprisingly did not yield any genuine target, thereby suggesting functional dominance of BjuGβ1. The triplicated BjuGβ isoforms showed a high degree of interaction strength and specificity with the identified target proteins, which are known to be involved in diverse biological functions in plants. qRT-PCR analysis further indicated that the expression of BjuGβ-target genes was developmentally regulated under various tissue types studied and showed a high degree of co-expression pattern with the BjuGβ genes, particularly during flower and silique development in B. juncea. Taken together, our data provides novel insights on pattern of expression and interaction specificity governing functional divergence of multiple Gβ subunit proteins in polyploid B. juncea.
The work was supported by the core grant of National Institute
of Plant Genome Research (NIPGR), India to NCB. RK was funded
with the Senior Research Fellowship by the University Grants
Commission (India). AS and IC were funded with Junior Research
Fellowship from Department of Biotechnology (DBT), India.
 
Date 2017-06-14T09:32:49Z
2017-06-14T09:32:49Z
2017
 
Type Article
 
Identifier Plant Physiology and Biochemistry, 118: 22-30
0981-9428
http://59.163.192.83:8080/jspui/handle/123456789/755
http://www.sciencedirect.com/science/article/pii/S0981942817301778
https://doi.org/10.1016/j.plaphy.2017.05.020
 
Language en_US
 
Publisher Elsevier B.V.