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Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana

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Title Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana
 
Creator Singh, Archita
Roy, Shradha
Singh, Sharmila
Das, Shabari Sarkar
Gautam, Vibhav
Yadav, Sandeep
Kumar, Ashutosh
Singh, Alka
Samantha, Sukanya
Sarkar, Ananda K.
 
Subject Plant physiology
Plant molecular biology
Arabidopsis thaliana
KANADI genes
Class III HOMEODOMAIN LEUCINE-ZIPPER (HD-ZIP III)
Phytohormonal
 
Description Accepted date: 19 April 2017
Both phytohormones and non-coding microRNAs (miRNAs) play important role in root development in Arabidopsis thaliana. Mature miR166/165 s, which are derived from precursor transcripts of concerned genes, regulate developmental processes, including leaf and root patterning, by targeting Class III HOMEODOMAIN LEUCINE-ZIPPER (HD-ZIP III) transcription factors (TFs). However, their regulation through hormones remained poorly understood. Here, we show that several phytohormones dynamically regulate the spatio-temporal expression pattern of miR166/165 and target HD-ZIP IIIs in developing roots. Hormone signaling pathway mutants show differential expression pattern of miR166/165, providing further genetic evidence for multilayered regulation of these genes through phytohormones. We further show that a crosstalk of at least six different phytohormones regulate the miR166/165, their target HD-ZIP IIIs, and KANADI (KANs). Our results suggest that HD-ZIP IIIs mediated root development is modulated both transcriptionally through phytohormones and KANs, and post-transcriptionally by miR166/165 that in turn are also regulated by the phytohormonal crosstalk.
We thank Department of Biotechnology (DBT, Govt. of India) for Ramalingaswami fellowship (BT/HRD/35/02/06/2008) grant to A.K.S. and UGC for fellowship to S.Y. Council of Scientific and Industrial Research (CSIR) provided fellowship to A.S., S.S., and V.G., and NIPGR provided internal funding, fellowship to A.K. is provided by SERB-NPDF (PDF/2015/000232) and is duly acknowledged. Fellowship to Al.S. is provided by DBT-RA and is duly acknowledged. Su.S. acknowledges NIPGR for internal grants and fellowship. SSD acknowledges Women Scientist-A (Wos-A) fellowship from Department of Science and Technology (DST), India WOS-A/LS- 1276/2014. We thank Dr. J.W. Reed, Dr. A.K. Nandi and ABRC for providing us with shy2-2, npr1-2 and other seed materials, respectively. We thank Suvakanta Barik for help with initial studies on cis-elements of MIR166s and HD-ZIP IIIs.
 
Date 2017-06-16T08:50:30Z
2017-06-16T08:50:30Z
2017
 
Type Article
 
Identifier Scientific Reports, 7(1): 3408
2045-2322
http://59.163.192.83:8080/jspui/handle/123456789/756
https://www.nature.com/articles/s41598-017-03632-w
10.1038/s41598-017-03632-w
 
Language en_US
 
Publisher Nature Publishing Group