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Characterization of mediator complex and its associated proteins from rice

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Title Characterization of mediator complex and its associated proteins from rice
 
Creator Samanta, Subhasis
Thakur, Jitendra K.
 
Subject Mediator complex
Mediator subunit
RNA Polymerase II
Transcription
Cofactor
Signaling
Rice
 
Description Accepted date: 17 June 2017
The Mediator complex is a multi-protein complex that acts as a molecular bridge conveying transcriptional messages from the cis element-bound transcription factor to the RNA Polymerase II machinery. It is found in all eukaryotes including members of the plant kingdom. Increasing number of reports from plants regarding different Mediator subunits involved in a multitude of processes spanning from plant development to environmental interactions have firmly established it as a central hub of plant regulatory networks. Routine isolation of Mediator complex in a particular species is a necessity because of many reasons. First, composition of the Mediator complex varies from species to species. Second, the composition of the Mediator complex in a particular species is not static under all developmental and environmental conditions. Besides this, at times, Mediator complex is used in in vitro transcription systems. Rice, a staple food crop of the world, is used as a model monocot crop. Realizing the need of a reliable protocol for the isolation of Mediator complex from plants, we describe here the isolation of Mediator complex from rice.
Research in our lab is funded by the grant BT/PR13009/
BPA/118/71/2015 from the Department of Biotechnology,
Government of India, to JKT and a NIPGR core grant. We thank
Mr. Mohd. Waseem for his help with the figures. SS acknowledges
Research Associateship from the Department of Biotechnology,
Government of India, and a Short-term Research Fellowship from
NIPGR.
 
Date 2017-06-19T11:05:11Z
2017-06-19T11:05:11Z
2017
 
Type Book chapter
 
Identifier Methods in Molecular Biology, 1629: 123-140
978-1-4939-7125-1
http://59.163.192.83:8080/jspui/handle/123456789/758
http://www.springerprotocols.com/Abstract/doi/10.1007/978-1-4939-7125-1_9
10.1007/978-1-4939-7125-1_9
 
Language en_US
 
Publisher Springer