Record Details

Transcription factor repertoire of necrotrophic fungal phytopathogen Ascochyta rabiei: predominance of MYB transcription factors as potential regulators of secretome

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Transcription factor repertoire of necrotrophic fungal phytopathogen Ascochyta rabiei: predominance of MYB transcription factors as potential regulators of secretome
 
Creator Verma, Sandhya
Gazara, Rajesh K.
Verma, Praveen K.
 
Subject plant–pathogen interaction
Ascochyta rabiei
necrotrophic fungi
transcription factors
secretome
cis-acting elements
 
Description Accepted date: 30 May 2017
Transcription factors (TFs) are the key players in gene expression and their study is
highly significant for shedding light on the molecular mechanisms and evolutionary
history of organisms. During host–pathogen interaction, extensive reprogramming of
gene expression facilitated by TFs is likely to occur in both host and pathogen.
To date, the knowledge about TF repertoire in filamentous fungi is in infancy. The
necrotrophic fungus Ascochyta rabiei, that causes destructive Ascochyta blight (AB)
disease of chickpea (Cicer arietinum), demands more comprehensive study for better
understanding of Ascochyta-legume pathosystem. In the present study, we performed
the genome-wide identification and analysis of TFs in A. rabiei. Taking advantage of
A. rabiei genome sequence, we used a bioinformatic approach to predict the TF
repertoire of A. rabiei. For identification and classification of A. rabiei TFs, we designed
a comprehensive pipeline using a combination of BLAST and InterProScan software.
A total of 381 A. rabiei TFs were predicted and divided into 32 fungal specific families of
TFs. The gene structure, domain organization and phylogenetic analysis of abundant
families of A. rabiei TFs were also carried out. Comparative study of A. rabiei TFs
with that of other necrotrophic, biotrophic, hemibiotrophic, symbiotic, and saprotrophic
fungi was performed. It suggested presence of both conserved as well as unique
features among them. Moreover, cis-acting elements on promoter sequences of earlier
predicted A. rabiei secretome were also identified. With the help of published A. rabiei
transcriptome data, the differential expression of TF and secretory protein coding genes
was analyzed. Furthermore, comprehensive expression analysis of few selected A. rabiei
TFs using quantitative real-time polymerase chain reaction revealed variety of expression
patterns during host colonization. These genes were expressed in at least one of the
time points tested post infection. Overall, this study illustrates the first genome-wide
identification and analysis of TF repertoire of A. rabiei. This work would provide the
basis for further studies to dissect role of TFs in the molecular mechanisms during
A. rabiei–chickpea interactions.
This study was supported by research grant (BT/PR7164/PBD/
16/1016/2012) of Department of Biotechnology, Government of
India and core grant from National Institute of Plant Genome
Research, New Delhi, India.
 
Date 2017-06-20T06:21:31Z
2017-06-20T06:21:31Z
2017
 
Type Article
 
Identifier Frontiers in Plant Sciences, 8: 1037
1664-462X
http://59.163.192.83:8080/jspui/handle/123456789/761
http://journal.frontiersin.org/article/10.3389/fpls.2017.01037/full
https://doi.org/10.3389/fpls.2017.01037
 
Language en_US
 
Publisher Frontiers Media S.A.