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Feeding behaviour of generalist pests on Brassica juncea: implication for manipulation of glucosinolate biosynthesis pathway for enhanced resistance

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Title Feeding behaviour of generalist pests on Brassica juncea: implication for manipulation of glucosinolate biosynthesis pathway for enhanced resistance
 
Creator Kumar, Pawan
Augustine, Rehna
Singh, Amarjeet Kumar
Bisht, Naveen C.
 
Subject Brassica juncea
MYB28
generalist pest
glucosinolates
plant defense
 
Description Accepted date: 15 June 2017
Differential accumulation of plant defense metabolites has been suggested to have important ecological consequence in the context of plant-insect interactions. Feeding of generalist pests on Brassica juncea showed a distinct pattern with selective exclusion of leaf margins which are high in glucosinolates. Molecular basis of this differential accumulation of glucosinolates could be explained based on differential expression profile of BjuMYB28 homologs, the major biosynthetic regulators of aliphatic glucosinolates, as evident from quantitative real-time PCR and promoter:GUS fusion studies in allotetraploid B. juncea. Constitutive over-expression of selected BjuMYB28 homologs enhanced accumulation of aliphatic glucosinolates in B. juncea. Performance of two generalist pests, Helicoverpa armigera and Spodoptera litura larvae on transgenic B. juncea plants were poor compared to wild-type plants in a no-choice experiment. Correlation coefficient analysis suggested that weight gain of H. armigera larvae was negatively correlated with gluconapin (GNA) and glucobrassicanapin (GBN) whereas that of S. litura larvae was negatively correlated with GNA, GBN and sinigrin (SIN). Our study explains the significance and possible molecular basis of differential distribution of glucosinolates in B. juncea leaves and shows the potential of over-expressing BjuMYB28 for enhanced resistance of Brassica crops against the tested generalist pests.
The work was funded by Department of Biotechnology-IYBA (BT/06/IYBA/2012) and the core-grant from National Institute of Plant Genome Research (NIPGR) to NCB. PK and RA were supported by fellowships from DBT and NIPGR respectively. We acknowledge Prof. Deepak Pental for his valuable suggestions and Dr. Prabodh Kumar Bajpai for his support with SPSS software. Technical assistance from Mr. Vinod Kumar and Mr. Amal Roul are acknowledged. The central instrumentation facility and plant growth facility at NIPGR are also acknowledged. The authors declare ‘No conflict of interest’ exists.
 
Date 2017-06-28T10:02:50Z
2017-06-28T10:02:50Z
2017
 
Type Article
 
Identifier Plant, Cell & Environment, 40(10): 2109 – 2120
1365-3040
http://59.163.192.83:8080/jspui/handle/123456789/763
http://onlinelibrary.wiley.com/doi/10.1111/pce.13009/abstract?systemMessage=Wiley+Online+Library+will+be+unavailable+on+Saturday+01st+July+from+03.00-09.00+EDT+and+on+Sunday+2nd+July+03.00-06.00+EDT+for+essential+maintenance.++Apologies+for+the+inconvenience.
10.1111/pce.13009
 
Language en_US
 
Publisher John Wiley & Sons