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A repeat length variation in myo-inositol monophosphatase gene contributes to seed size trait in chickpea

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Title A repeat length variation in myo-inositol monophosphatase gene contributes to seed size trait in chickpea
 
Creator Dwivedi, Vikas
Parida, Swarup K.
Chattopadhyay, Debasis
 
Subject chickpea
myo-inositol
monophosphatase gene
Cicer arietinum L.
 
Description Accepted date: 26 May 2017
Chickpea (Cicer arietinum L.) is the third most important food legume crop. Seed size is the most economically important trait for chickpea. To understand the genetic regulation of seed size in chickpea, the present study established a three-way association of CT repeat length variation of a simple sequence repeat (SSR) in myo-inositol monophosphatase gene (CaIMP) with seed weight and phytic acid content by large scale validation and genotyping in a set of genetically diverse germplasm accessions and two reciprocal intra-specific mapping populations. Germplasms and mapping individuals with CT repeat-length expansion in the 5' untranslated region of CaIMP exhibited a pronounced increase in CaIMP protein level, enzymatic activity, seed-phytate content and seed weight. A chickpea transient expression system demonstrated this repeat-length variation influenced the translation of CaIMP mRNA, apparently by facilitating translation initiation. Our analyses proposed that the SSR marker derived from 5' UTR of a CaIMP gene is a promising candidate for selection of seed size/weight for agronomic trait improvement of chickpea.
The project is funded by the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India (BT/HRD/35/01/03/2014). VD acknowledges DBT for fellowship. Authors acknowledge help of Dr. Manoj Majee for assay of enzymatic activity.
 
Date 2017-07-11T11:33:20Z
2017-07-11T11:33:20Z
2017
 
Type Article
 
Identifier Scientific Reports, 7(1): 4764.
2045-2322
http://59.163.192.83:8080/jspui/handle/123456789/766
https://www.nature.com/articles/s41598-017-05332-x
10.1038/s41598-017-05332-x
 
Language en_US
 
Publisher Nature Publishing Group